慢性肾脏病患者血清HIF-1α的表达及临床意义

Expression and Significance of Serum HIF-1α in Patients with Chronic Kidney Disease

目的研究慢性肾脏病(chronic kidney disease,CKD)患者血清中的缺氧诱导因子-1α(hypoxia inducible factors-1α,HIF-1α)的表达情况,分析其与血清纤维化指标Ⅲ型前胶原氨基末端肽(typeⅢprocollagen amino terminal peptide,PⅢNP)、CKD患者临床指标的关系,探讨HIF-1α在CKD中的临床应用价值。方法随机抽取63例CKD患者为研究对象,根据CKD分期对研究对象进行分组,CKD1~2期30例患者为A组,CKD3期17例患者为B组,CKD4~5期16例患者为C组,ELISA法检测入组患者血清中HIF-1α及PⅢNP的表达,同时收集入组患者的临床资料,包括姓名、性别、年龄、血常规、肝功能、肾功能、离子、24 h尿蛋白。结果 HIF-1α与PⅢNP、血肌酐(serum creatinine,Scr)、血尿素氮(blood urea nitrogen,BUN)、γ-谷氨酰转肽酶(γ-glutamyltranspeptidase,γ-GT)呈正相关(P <0.05),而与肾小球滤过率(glomerular filtration rate,GFR)、血红蛋白(hemoglobin,HB)、血钙呈负相关(P <0.05),与中性粒细胞与淋巴细胞比值(neutropil-to-lymphocyte ratio,NLR)、血小板与淋巴细胞比值(platelet-to-lymphocyte ratio,PLR)、白蛋白(albumin,ALB)、血磷、24 h尿蛋白无相关性(P> 0.05)。结论 HIF-1α与血清纤维化指标PⅢNP、肾功能、CKD并发症相关,可能参与CKD疾病的进展,可作为评价CKD的指标之一。

Objective To study the expression of HIF-1αin the serum of CKD patients,analyze the relationship between it and serum fibrosis index PⅢNP,and the clinical indicators of CKD patients,and explore the clinical application value of HIF-1αin CKD.Methods A total of 63 CKD patients were randomly selected as the research object,and the research objects were grouped according to the CKD stage.30 patients in CKD1-2 were in group A,17 patients in CKD3 were in group B,and 16 patients in CKD4-5 were in group C.ELISA method was used to detect the expression of HIF-1αand PⅢNP in the serum of the enrolled patients,and the clinical data of the enrolled patients were collected,including gender,age,blood routine,liver function,renal function,ions,and 24-hour urine protein.Results HIF-1αwas positively correlated with PⅢNP,Scr,BUN,γ-GT(P<0.05),negatively correlated with GFR,HB,Blood calcium(P<0.05),There was no correlation with NLR,PLR,ALB,Phosphorus and 24-hour urine protein(P>0.05).Conclusion HIF-1αis associated with serum fibrosis indexes,renal function,CKD complications,and may be involved in the progression of CKD disease,which can be used as one of the indicators for evaluating CKD.