糖尿病性视网膜病变患者房水中炎性因子的作用

Expressions of Inflammatory Factors in Aqueous Humor of Patientswith Diabetic Retinopathy and Their Significances

目的探究糖尿病性视网膜病变患者房水中血管内皮生长因子(vascular endothelial growth factor,VEGF)、白介素-1β(interleukin-1β,IL-1β)、白介素-6(interleukin-6,IL-6)、白介素-8(interleukin-8,IL-8)及肿瘤坏死因子-α(tumor necrosis factor,TNF-α)等炎性因子水平,并分析其在糖尿病性视网膜病变中的作用与意义。方法选取2019年1月至2020年1月我院收治的2型糖尿病性视网膜病变50例为A组,其中2型糖尿病史≤5年28例;2型糖尿病史>5年22例。选取2型糖尿病无视网膜病变50例为B组,另选取同期行体检的50例健康受试者为C组。采集所有受试者的房水0.2 mL,检测房水样本中VEGF、IL-1β、IL-6、IL-8及TNF-α水平,并分析各组炎性因子水平差异性。结果A、B两组患者的房水VEGF、IL-1β、IL-6、IL-8及TNF-α水平均显著高于C组,差异具有统计学意义(P<0.05)。A组患者房水VEGF、IL-1β、IL-6、IL-8及TNF-α水平显著高于B组,差异具有统计学意义(P<0.05)。A组中糖尿病史>5年患者房水VEGF、IL-1β、IL-6、IL-8及TNF-α水平均显著高于糖尿病史≤5年组患者(P<0.05)。房水中VEGF水平与IL-1β、IL-6、IL-8及TNF-α均呈正相关(r值分别为0.471、0.376、0.464、0.360,P<0.05)。IL-1β水平与IL-6、IL-8及TNF-α均呈正相关(r值分别为0.465、0.512、0.389,P<0.05)。IL-6水平与IL-8及TNF-α均呈正相关(r值分别为0.366、0.267,P<0.05)。IL-8水平与TNF-α呈正相关(r=0.416,P<0.05)。结论房水VEGF、IL-1β、IL-6、IL-8及TNF-α水平升高与糖尿病性视网膜病变及病情进展密切相关。各炎性因子间存在显著正相关性,炎性因子间可能共同发挥作用参与糖尿病性视网膜病变的发生和病情进展。

Objective To investigate the expressions of vascular endothelial growth factor(VEGF),interleukin-1β(IL-1β),interleukin-6(IL-6),interleukin-8(IL-8)and tumor necrosis factor-α(TNF-α)in aqueous humor of patients with diabetic retinopathy,and to analyze their role and significance in diabetic retinopathy.Methods 50 cases of type 2 diabetic retinopathy admitted to our hospital from January,2019 to January,2020 were selected as group A,of which 28 cases had a history of type 2 diabetes less than 5 years,while 22 cases had a history of type 2 diabetes greater than 5 years.Fifty patients with type 2 diabetes without retinopathy were selected as group B,and 50 healthy subjects who underwent physical examination during the same period were selected as group C.We collected 0.2mL aqueous humor from all subjects,then detected the levels of VEGF,IL-1β,IL-6,IL-8 and TNF-αin the aqueous samples,and analyzed the differences in the levels of inflammatory factors across these groups.Results The expression levels of VEGF,IL-1β,IL-6,IL-8 and TNF-αin aqueous humor of patients in group A and B were significantly higher than those in group C(P<0.05).The expression levels of VEGF,IL-1β,IL-6,IL-8 and TNF-αin aqueous humor of patients in group A were significantly higher than those in group B(P<0.05).The expression levels of VEGF,IL-1β,IL-6,IL-8 and TNF-αin aqueous humor of patients with diabetes history greater than 5 years in group A were significantly higher than those of patients with diabetes history less than or equal to 5 years(P<0.05).The VEGF level in aqueous humor was positively correlated with IL-1β,IL-6,IL-8 and TNF-α(r=0.471,0.376,0.464 and 0.360,respectively,P<0.05).The level of IL-1βwas positively correlated with IL-6,IL-8 and TNF-α(r=0.465,0.512 and 0.389 respectively,P<0.05).The level of IL-6 was positively correlated with IL-8 and TNF-α(r=0.366,0.267,P<0.05).The level of IL-8 was positively correlated with TNF-α(r=0.416,P<0.05).Conclusion The expressions of VEGF,IL-1β,IL-6,IL-8 and TNF-αin aqueous humor are closely related to diabetic retinopathy and disease progression.There are significant positive correlations among inflammatory factors,which may play a joint role in the occurrence and progression of diabetic retinopathy.