高温对感染Wolbachia米蛾体内保护酶和生殖的影响

Effects of High Temperature on Protective Enzymes and Reproduction in Infected with Wolbachia

为揭示高温处理对感染Wolbachia米蛾生殖发育及生理特性的影响,本研究测定不同温度和时间组合处理条件下米蛾体内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)三种保护酶的活性,同时在室温25℃和高温31℃下,观察米蛾的孵化率、化蛹率、羽化率、雌蛾率、产卵量和发育历期等指标,统计米蛾的生物学特性。结果表明:在室温和高温条件下,米蛾的孵化率、化蛹率、羽化率、雌蛾率均无显著差异,但严重影响了米蛾成虫的产卵量和发育历期。而在31℃处理条件下,其体内SOD含量逐渐升高,35℃条件CAT含量急剧下降,在25℃处理条件下其保护酶系统处于平衡状态。高温会影响感染Wolbachia米蛾的发育历期,产卵量和繁殖量并且会破坏米蛾体内保护酶的平衡,高温对感染Wolbachia米蛾的生殖方式影响不显著。

In order to reval the effect of high temperature on the reproductive development and physiological characteristics of rice moth infected with Wolbachia,the activities of superoxide dismutase(SOD),catalase(CAT)and peroxidase(POD)protective enzymes were measured under different temperature and time combinations,to explore the influence of high temperature on rice moth protective enzyme system,at the same time under room temperature 25℃and 31℃,to observe the hatchability of rice moth,pupa rate and eclosion rate,rate of female moth,indices such as spawning and development period,estimated the biological characteristics of the rice moth.The results showed that there was no significant difference in hatching rate,pupation rate,emergence rate and female moth rate under room temperature and high temperature conditions,but it had a serious impact on the amount of eggs laid by the adults and the development duration of the moth.At 31℃processing conditions,its body SOD content increased,content of 35℃condition CAT fell sharply,under the condition of 25℃to deal with the protective enzyme system in a state of balance.high temperature would affect the developmental duration,spawning quantity and fecundity of the infected Wolbachia,and would also destroy the balance of protective enzymes in the body of the infected Wolbachia,while high temperature had little effect on the reproductive pattern of the infected Wolbachia.