AmpC ompK35在亚胺培南诱导肺炎克雷伯菌耐药中的相关性研究

Correlation Between AmpC and Ompk35 in Imipenem Induced Resistance of Klebsiella Pneumoniae

目的:探讨AmpC、ompK35在亚胺培南诱导肺炎克雷伯菌耐药中的作用。方法:2015年1月至2020年1月,收集我院分离培养的肺炎克雷伯菌200株,其中耐亚胺培南菌株50株(A组),不耐亚胺培南菌株150株(B组),采用PCR方法检测AmpC、ompK35基因表达,Western blot检测AmpC、ompK35蛋白表达。结果:A组AmpC基因、蛋白相对表达量分别为(1.658±0.122)和(1.501±0.106),明显高于B组(P<0.05);A组ompK35基因、蛋白相对表达量分别为(0.712±0.110)和(0.611±0.107),明显低于B组(P<0.05);AmpC基因与ompK35基因呈负相关(r=-0.329,P<0.05);AmpC蛋白与ompK35蛋白呈负相关(r=-0.435,P<0.05)。结论:耐亚胺培南肺炎克雷伯菌AmpC表达量升高,ompK35表达量降低,两者可能在耐药机制中有重要作用。

Objective:To investigate the role of AmpC and ompk35 in imipenem induced resistance of Klebsiella pneumoniae.Methods:A total of 200 strains of Klebsiella pneumoniae isolated and cultured in our hospital from January 2015 to January 2020 were collected,among which 50 strains were resistant to imipenem(group A),150 strains were not resistant to imipenem(group B),the gene expression of AmpC and ompk35 was detected by PCR,and the protein expression of AmpC and ompk35 was detected by Western blot.Results:The relative expression of AmpC gene and protein in group A were(1.658±0.122)and(1.501±0.106),which were significantly higher than that in group B(P<0.05);The relative expression of ompk35 gene and protein in group A were(0.712±0.110)and(0.611±0.107),which were significantly lower than that in group B(P<0.05);The correlation between AmpC gene and ompk35 gene was negative(r=-0.329,P<0.05);the correlation between AmpC protein and ompk35 protein was negative(r=-0.435,p<0.05).Conclusion:The expression of AmpC in imipenem resistant Klebsiella pneumoniae increase,and the expression of ompk35 decrease.They may play an important role in the mechanism of drug resistance.