摘要
利用枯草芽孢杆菌XF-1的发酵液处理十字花科作物大白菜、油菜、红菜苔、大头菜、萝卜、水果萝卜和花椰菜,通过HPLC检测了各作物根部的异硫氰酸苯乙酯含量和黑芥子酶活性。结果表明:未处理的萝卜体内的异硫氰酸苯乙酯含量最高,达2.369 mg/g;经XF-1处理后,各作物根部的异硫氰酸苯乙酯含量均有所提高,以大头菜的提高幅度最大,增长率达122.09%;未处理的水果萝卜体内的黑芥子酶活性最高,达4.587 U/mg;处理后各作物的黑芥子酶活性均有不同程度的提高,以萝卜的增幅最大,增长率达68.33%。说明XF-1可能参与了十字花科作物硫代葡萄糖苷的代谢,从而提高了各作物对根肿病的抗性。
Phenethyl isothiocyanate content and myrosinase activity of cruciferous crops,such as Chinese cabbage,canola,purple flowering stalk,rutabaga,radish,fruit radish and cauliflower were tested by HPLC after the crops were drenched with Bacillus subtilis XF-1 ferment solution.The results showed that phenethyl isothiocyanate was quite different among the crops untreated,and radish had the highest content of 2.369 mg/g based on HPLC analysis.The content of the roots increased to some degree after XF-1 treatment,Rutabaga had the largest increase(122.09%).The untreated fruit radish had the highest myrosinase activity(4.587 U/mg).The activity of the crops increased after XF-1 treatment,and radish had the largest increase(68.33%).These results implied that XF-1 might be involved in the glucosinolate metabolism,thus clubroot disease resistance of cruciferous crops could be impoved.
作者
张照然
何朋杰
李兴玉
何鹏飞
吴毅歆
范黎明
何月秋
ZHANG Zhao-ran;HE Peng-jie;LI Xing-yu;HE Peng-fei;WU Yi-xin;FAN Li-ming;HE Yue-qiu(Fushun Academy of Agricultural Sciences,Fushun 113300,China;College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China;College of Foundation and Information Engineering,Yunnan Agricultural University,Kunming 650201,China;College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China;National and Local Joint Engineering Research Center for Screening and Application of Microbial Strains,Kunming 650217,China)
出处
《江西农业学报》
CAS
2021年第1期23-27,共5页
Acta Agriculturae Jiangxi
基金
科技部重点研发计划项目(2018YFD0201200-05)。