LncRNA LINC00987通过靶向miR-375抑制肺癌细胞的增殖、侵袭和迁移

LncRNA LINC00987 inhibits the proliferation,invasion and migration of lung cancer cells by targeting miR-375

目的探讨lncRNA LINC00987对肺癌细胞增殖、侵袭和迁移的影响。方法以正常肺泡上皮细胞HPAEPIC为对照,qRT-PCR检测肺癌细胞株A549、HCC827和H1299中LINC00987表达量,选择表达差异较大的A549细胞进行后续实验。将A549细胞分为pcDNA-control组、pcDNA-LINC00987组、LINC00987(WT)+miR-control组、LINC00987(WT)+miR-375组、LINC00987(MUT)+miR-control组、LINC00987(MUT)+miR-375组、si-control组、si-LINC00987组、miR-control组、miR-375组、anti-miR-control组、anti-miR-375组、pcDNA-LINC00987+miR-control组和pcDNA-LINC00987+miR-375组。CCK8法和Transwell实验分别检测细胞增殖、侵袭和迁移能力,双荧光素酶报告系统验证LINC00987和miR-375的调控关系。结果与正常肺泡上皮细胞HPAEPIC相比,在肺癌A549、HCC827和H1299细胞中LINC00987表达量均显著降低(P<0.05);pcDNA-LINC00987组可抑制A549细胞增殖、侵袭和迁移;LINC00987靶向抑制miR-375的表达;miR-375组可促进A549细胞增殖、侵袭和迁移,anti-miR-375组结果反之;pcDNA-LINC00987+miR-375组提高A549细胞增殖、侵袭和迁移。结论LncRNA LINC00987靶向miR-375抑制肺癌A549细胞增殖、侵袭和迁移。LINC00987可能是肺癌的潜在分子靶点。

Objective To investigate the effect of lncRNA LINC00987 on proliferation,invasion and migration of lung cancer cells.Methods Taking normal alveolar epithelial cell HPAEPIC as the control,the expression levels of LINC00987 in lung cancer cell lines A549,HCC827 and H1299 were detected by qRT-PCR.A549 cells are divided into pcDNA-control group,pcDNA-LINC00987 group,LINC00987(WT)+miR-control group,LINC00987(WT)+miR-375 group,LINC00987(MUT)+miR-control group,LINC00987(MUT)+miR-375 group,si-control group,si-LINC00987 group,miR-control group,miR-375 group,anti-miR-control group,anti-miR-375 group,pcDNA-LINC00987+miR-control group and pcDNA-LINC00987+miR-375 group.The proliferation,invasion and migration abilities of A549 cells were determined by CCK8 and Transwell assay,respectively.The relationship between LINC00987 and miR-375 was verified by dual-luciferase reporter assay system.Results Compared with normal alveolar epithelial cells HPAEPIC,the expression levels of LINC00987in lung cancercell lines A549,HCC827 and H1299were decreased significantly(P<0.05),and the following experiments were carried out on A549 cells with remarkably different expression levels.pcDNA-LINC00987 group inhibited the proliferation,invasion and migration of A549 cells.LINC00987 targeted and inhibited the expression of miR-375.The miR-375 group could promote the proliferation,invasion and migration of A549 cells,while the anti-miR-375 group had the opposite result;the pcDNA-LINC00987+miR-375 group promoted the proliferation,invasion and migration of A549 cells.Conclusion LncRNA LINC00987 inhibits proliferation,invasion and migration of lung cancer A549 cells by targeting miR-375.LINC00987 may be a potential molecular target for lung cancer.