骨髓间充质干细胞干预治疗骨质疏松症中的骨组织生物力学性能

Biomechanical properties of bone tissue in the treatment of osteoporosis with bone marrow mesenchymal stem cells

目的以雷洛昔芬干预治疗骨质疏松为对照,用生物力学性能指标验证骨髓间充质干细胞(bone mesenchymal stem cells,BMSCs)干预治疗骨质疏松动物的效果。方法取6月龄雌性SD大鼠88只,随机分为骨质疏松动物模型组22只,正常对照组22只,雷洛昔芬干预组22只,骨髓间充质干细胞干预组22只。以摘除大鼠卵巢的方法建立骨质疏松动物模型。动物饲养4周后,分别以骨髓间充质干细胞和雷洛昔芬对骨质疏松动物进行干预治疗。于给药6周后检测各组大鼠血清Ca、P、碱性磷酸酶(alkaline phosphatase,ALP),并取胫骨进行剪切实验,取股骨进行冲击实验,测试肱骨的骨密度(bone mineral density,BMD)。结果骨髓间充质干细胞干预组肱骨BMD,血清Ca、ALP,胫骨各项剪切力学性能指标和股骨各项冲击力学性能指标大于模型组及雷洛昔芬干预组,其血清P小于模型组及雷洛昔芬干预组(P<0.05)。结论骨质疏松动物通过以骨髓间充质干细胞干预后,肱骨BMD,血清Ca、ALP,骨的强度和韧性可以得到一定恢复。

Taking raloxifene intervention in the treatment of osteoporosis as a control,biomechanical performance indicators are used to verify the effect of bone marrow mesenchymal stem cells(BMSCs)in the treatment of osteoporosis in animals.Methods 88 SD rats were randomly divided into 22 osteoporosis animal model group,22 normal control group,22 raloxifene intervention group,and 22 bone marrow mesenchymal stem cell intervention group.The osteoporosis animal model was established by removing the ovaries of rats.After the animals were raised for 4 weeks,bone marrow mesenchymal stem cells and raloxifene were used to intervene in osteoporotic animals.After 6 weeks of administration,the serum Ca,P,alkaline phosphatase(ALP)of each group of rats were measured,and tibia was taken for shearing experiment,femur was taken for impact experiment,and the bone mineral density(BMD)of humerus was tested.).Results The humerus BMD,serum Ca,ALP,tibia and various impact mechanical performance indexes of the bone marrow mesenchymal stem cell intervention group were greater than those of the model group and the raloxifene intervention group,and the serum P was lower than the model group and Raloxifene intervention group(P<0.05).Conclusions After the intervention of bone marrow mesenchymal stem cells in osteoporotic animals,humerus BMD,serum Ca,ALP,bone strength and toughness can be restored to a certain extent.