表面增强拉曼光谱快速检测食品基质中毒物

Surface Enhanced Raman Spectroscopy Applied in Rapid Detection of Food Poisoning

目的建立常见食品基质中氰化物等五种毒物的拉曼光谱快速分析方法。方法采用水、乙腈及乙酸乙酯作为提取剂对饮用水、苹果以及烤鸭三种常见食品基质中氰化物、百草枯、毒死蜱、甲拌磷以及敌鼠钠五种毒物进行提取,并使用表面增强拉曼光谱法检测。结果实验表明,每种毒物均有其独特的拉曼信号,这些特征信号可作为其定性的判别依据,氰化物在饮用水、苹果以及烤鸭这三种基质中的检出限均不大于1μg/mL(g);百草枯的检出限在0.05~0.1μg/mL(g)之间;毒死蜱、甲拌磷的检出限在0.05~1μg/mL(g)之间;敌鼠钠检出限在0.1~1μg/mL(g)之间,均满足现场检测需求。结论该方法操作简单且分析速度快,为毒物的快速筛查提供了较好的解决方案。

Objective To establish a method of Raman spectroscopic analysis about fi ve poisons likely envenomed into common foods.Methods Five toxicants/poisons of cyanide,paraquat,chlorpyrifos,thimet and diphacinone were respectively spiked into three common food materials(drinking water,smashed apple and roast duck fl esh),having them extracted with the relative water,acetonitrile and ethyl acetate.Surface enhanced Raman spectroscopy was adopted to detect the extracted toxicants.Results Each toxicant has shown its own unique Raman signals that are capable of being used as the basis for qualitative discrimination.The detection limits were no more than 1μg/g for cyanide in drinking water,fruit and roast duck fl esh,demonstrating the individual 0.05~0.1μg/mL(g),0.05~1μg/mL(g)and 0.1~1μg/mL(g)for paraquat,chlorpyrifos/thimet and diphacinone,respectively.The results proved that the adopted method is eligible for rapid detection on scene.Conclusion The method established here is of simple operation,fast analysis speed,having provided a correct solution for rapid detection/screening of poisons/toxicants.