p16与高危型人乳头瘤病毒感染关系及在不同宫颈病变患者中表达

Relationship between p16 protein expression and HR-HPV infection and the analysis of the expression level of p16 in patients with different cervical lesions

目的:对比不同宫颈病变患者p16表达及与高危型人乳头瘤病毒(HR-HPV)感染关系。方法:收集2017年6月-2019年6月本院收治的宫颈疾病患者104例,其中HR-HPV阳性高级别鳞状上皮内瘤变(HSIL)60例,HR-HPV阳性宫颈炎15例,HR-HPV阴性宫颈炎15例,HR-HPV阳性宫颈癌(CC)14例。检测患者宫颈活检病理组织中P16蛋白表达水平。结果:13种高危型HPV分型中,p16蛋白表达阳性率均≥50%,其中阳性率≥80%有7种,p16蛋白表达总阳性率为84.3%;P16蛋白表达水平越高HPV感染率越高(P<0.05);不同宫颈病变者p16蛋白表达水平有差异(P<0.05),宫颈病变越严重p16蛋白表达水平越高,CINⅢ级患者p16阳性率(95.8%%)高于CINⅡ级患者(75.0%)(P<0.05)。结论:宫颈病变患者p16表达水平与宫颈病变程度及HR-HPV感染有关,检测p16表达水平可能有助于宫颈疾病的诊断。

Objective:To compare p16 protein expression level of patients with different cervical lesions,and to investigate the relationship between p16 expression of patients and high-risk human papillomavirus(HR-HPV)infection.Methods:104 patients with cervical diseases were enrolled in this study between June 2017 and June 2019.Among them,there were 60 cases with HR-HPV positive high-grade squamous intraepithelial lesions(HSIL),15 cases with HR-HPV positive cervicitis,15 cases with HR-HPV negative cervicitis and 14 cases with HR-HPV positive cervical cancer(CC).The expression level of P16 protein in cervical biopsy pathological tissues of these patients was detected.Results:Among the patients with 13 kinds of high-risk HPV infection,the positive expression rate of p16 protein in their cervical biopsy pathological tissues was more than 50%.The positive expression rate of p16 protein of the patients with 7 kinds of high-risk HPV infection was more than 80%.The total positive expression rate of p16 protein of these 104 patients was 84.3%.The higher of the expression level of P16 protein was,the higher of HPV infection rate was(P<0.05).There was significant differences in expression levels of p16 protein of the patients with different cervical lesions(P<0.05).The cervical lesion was more severe,the expression level of P16 protein was the higher(P<0.05).The p16 positive rate of patients with CINⅢ(95.8%)was significant higher than that(75.0%)of women with CINⅡ(P<0.05).Conclusion:The expression level of p16 is related to the severity of cervical lesions and HPV infection.The detection of p16 expression level is helpful for diagnosing cervical diseases.