ORAOV1和Vasohibin1在乳腺癌中的表达及其临床意义

Expression of ORAOV1 and Vasohibin1 in infiltrating breast carcinoma, and their clinicopathological significance

目的:检测乳腺浸润性乳腺癌(infiltrating breast carcinoma,IBC)中口腔癌过表达蛋白1(oral cancer overexpressed protein 1,ORAOV1)和血管生成抑制因子1(vasohibin1,VASH1)的表达情况及其相互之间的关系,并分析它们在IBC中的临床意义。方法:通过免疫组织化学方法检测239例IBC和100例相应的正常乳腺组织中ORAOV1和VASH1蛋白的阳性表达情况。结果:在IBC组织中,ORAOV1和VASH1蛋白的阳性率分别为58.2%和56.9%;对照组中,ORAOV1和VASH1蛋白的阳性率分别为13.0%和12.0%,其表达差异在2组间均有统计学意义(P<0.01)。ORAOV1和VASH1蛋白阳性率的差异在IBC病人肿瘤组织的不同分化程度之间、淋巴结转移与否之间、肿瘤大小之间及不同TNM分期之间差异均有统计学意义(P<0.05~P<0.01);且ORAOV1蛋白的阳性率与VASH1蛋白的阳性率之间呈正相关关系(P<0.01)。在IBC病人中ORAOV1和VASH1蛋白阳性组术后的生存时间明显低于阴性组(P<0.05~P<0.01)。多因素回归分析显示阳性表达的ORAOV1和VASH1蛋白、肿瘤组织的大小及TNM分期高低均是影响IBC病人术后生存时间的独立预后因子(P<0.05~P<0.01)。结论:表达异常增高的ORAOV1和VASH1可能参与IBC的发生、发展,并促进其浸润和转移;在IBC病人肿瘤组织中联合检测ORAOV1和VASH1蛋白的表达情况对预测其发展和预后均有重要意义。

Objective:To investigate the expression levels of oral cancer overexpressed protein 1(ORAOV1)and vasohibin 1(VASH1)in infiltrating breast carcinoma(IBC),their relationship with each other,and their clinical significance in IBC.Methods:The positive expression of ORAOV1 and VASH1 protein in 239 cases of IBC tissue and 100 cases of normal mammary tissue were detected using immunohistochemistry.Results:The positive rates of ORAOV1 and VASH1 protein in IBC tissue and control tissues were 58.2%&56.9%and 13.0%&12.0%,respectively,and the expression differences of whose were statistically significant between two groups(P<0.05).The differences of the positive expression rates of ORAOV1 and VASH1 protein among IBC patients with different degrees of differentiation,lymph node metastasis,tumor size and TNM stage were statistically significant(P<0.05 to P<0.01).The positive rate of ORAOV1 protein was positively correlated with the positive rate of VASH1 protein(P<0.01).The overall survival time of IBC′s patients with positive expression ORAOV1 and VASH1 was lower than that of IBC′s patients with negative expression of ORAOV1 and VASH1(P<0.01).The results of multivariate COX regression analysis showed that the positive expression of ORAOV1 and VASH1 protein,tumor size and TNM stages were the independent prognostic factor affecting postoperative survival time of IBC patients.Conclusions:The ORAOV1 and VASH1 with abnormally high expression may participate in the occurrence and development of IBC,and promote its infiltration and metastasis.The combined detection of ORAOV1 and VASH1 protein expression in tumor tissues of IBC patients is of great significance for the prediction of development and prognosis.