肺腺癌表皮生长因子受体基因突变与组织病理学的相关性研究

Study on the correlation between the epidermal growth factor receptor gene mutation and histopathological characteristics in lung adenocarcinoma

目的:研究肺腺癌(LAC)表皮生长因子受体(EGFR)基因突变与组织病理学的相关性。方法:选取179例行LAC根治手术病人的癌组织石蜡标本,采用扩增阻滞突变系统检测LAC组织中EGFR基因突变及突变丰度情况,分析EGFR基因突变及突变丰度与临床病理特征的相关性。结果:179例LAC病人中,EGFR基因突变者共108例(60.34%),其中以19号外显子和21号外显子突变为主。不同年龄、淋巴结转移情况、胸膜浸润程度的LAC病人癌组织中EGFR基因突变差异无统计学意义(P>0.05),不同性别、吸烟情况、肿瘤直径、临床分期、组织学亚型的LAC病人癌组织中EGFR基因突变和突变丰度差异均有统计学意义(P<0.05~P<0.01)。结论:LAC病人EGFR基因突变与性别、吸烟情况、肿瘤直径、临床分期、组织学亚型相关,女性、肿瘤直径较小、不吸烟者、临床分期较早、腺泡状肺腺癌有较高的EGFR基因突变率。

Objective:To investigate the correlation between epidermal growth factor receptor(EGFR)gene mutation and histopathology in lung adenocarcinoma(LAC).Methods:The paraffin-embedded specimens of 179 patients treated with LAC radical surgery were investigated.The mutation and abundance of EGFR gene in 179 LAC tissues were detected using amplification refractory mutation system(ARMS),and the correlation between the mutation of EGFR gene and clinicopathological characteristics was analyzed.Results:Among 179 patients with LAC,108(60.34%)cases with EGFR gene mutations were found,and the exon 19 and 21 were the main mutations.The differences of EGFR gene mutations among LAC patients with different age,lymph node metastasis and invasive degree were not statistically significant(P>0.05),and the differences of mutation and abundance of EGFR gene among patients with different gender,smoking status,tumor diameter,clinical stage and histological subtype were statistically significant(P<0.05 to P<0.01).Conclusions:The EGFR gene mutation is associated with the gender,smoking status,tumor diameter,clinical stage and histological subtype.The EGFR mutation in female,small tumor diameter,nonsmokers,early clinical stage and alveolar LAC patients is high.