多重聚合酶链式反应技术在食源性致病菌检测上的应用研究进展

Application of multiplex polymerase chain reaction in detection of foodborne pathogens

多重聚合酶链式反应(polymerase chain reaction,PCR)技术是在常规PCR技术基础上发展起来的,其反应原理、反应试剂和操作过程与常规PCR相同,区别在于多重PCR技术在同一个反应体系中加入2对或2对以上引物,分别扩增不同的模板,得到不同的目的片段。多重PCR技术在食源性致病菌检测中具有高通量、节约成本的优势,在实际检测中具有很好的发展前景。该文介绍了我国现行食源性致病菌检测方法,总结了多重PCR技术在食源性致病菌检测中的研究进展以及在标准制定、商业化应用上的现状,提出了多重PCR技术在食源性致病菌检测中的技术要点和未来研究方向,以期对多重PCR技术在食源性致病菌检测中的进一步发展和研究提供参考。

Multiplex PCR technology is developed on the basis of conventional PCR technology,its reaction principle,reaction reagent and operation process are the same as conventional PCR.The difference is that multiplex PCR technology adds two or more pairs of primers in the same reaction system to amplify different templates and get different target fragments.Multiplex PCR technology has the advantages of high throughput and cost saving in the detection of foodborne pathogens,so it has a good development prospect in the actual detection.This paper summarizes the research progress of multiplex PCR technology in the detection of foodborne pathogens and its current situation in standard formulation and commercial application,and puts forward the technical points and future research directions of multiplex PCR technology in the detection of foodborne pathogens.