贵州水族人群11个Y-SNP位点的多态性及法医学应用

Polymorphism and Forensic Application of 11 Y-SNP in Guizhou Shui Ethnic Group

目的调查贵州水族人群Y染色体上11个SNP位点的频率分布情况,并探讨该人群与其他人群的遗传关系,评价其法医学应用价值。方法采用微测序技术对贵州地区180例水族男性无关个体样本11个Y-SNP位点进行复合扩增检测。采用直接计数法计算单倍群频率,应用Multi-variate statistical package(MVSP)对贵州水族及参考人群进行主成分分析(principal component analysis,PCA),采用Arlequin v3.5软件计算贵州水族与其他群体的Fst遗传距离,使用MEGA 4.0软件根据Fst值绘制系统发生树。结果共观察到6种Y染色体单倍群,其中O-M175单倍群的分布频率最高(71.11%),其次为C-M130单倍群(25.00%)、D-M174单倍群(3.89%)。O单倍群中分布频率较高的是O1b-M268(31.11%)、O2a2-IMS-JST021354(28.33%)。贵州水族与同语族的贵州仡佬族父系的亲缘关系最近。结论贵州水族Y-SNP单倍群分布具有一定特异性,可为法医学族群地域推断提供基础数据。

Objective To investigate the frequency distribution features of 11 Y-SNP of Guizhou Shui ethnic group,explore its genetic relationship with other ethnic groups and evaluate its forensic application value.Methods Multiplex amplification of the 11 Y-SNP of samples of 180 unrelated male individuals from Guizhou Shui ethnic group was performed with microsequencing technique.The frequency of haplogroup was calculated by direct counting method,and principal component analysis(PCA)of Guizhou Shui ethnic group and reference ethnic groups was performed by using Multi-variate statistical package(MVSP).The Fst genetic distance between Guizhou Shui ethnic group and other ethnic groups was calculated with Arlequin v3.5.The phylogenetic tree was established with MEGA 4.0 software according to the Fst value.Results Six types of Y chromosome haplogroups were observed in total.Among which,the distribution frequency of O-M175 haplogroup was the highest(71.11%),followed by C-M130(25.00%),and D-M174(3.89%).O1b-M268(31.11%)and O2a2-IMS-JST021354(28.33%)had a relatively high distribution frequency in O haplogroup.The paternal relationship between Guizhou Shui ethnic group and Guizhou Gelao ethnic group in the same language group was the closest.Conclusion The distribution of Y-SNP haplogroup of the Shui ethnic group in Guizhou has certain specificity,which can provide basic data for forensic biogeographic inference.