α6/α3β4乙酰胆碱受体在非洲爪蟾卵母细胞中的表达

Expression ofα6/α3β4 Nicotinic Acetylcholine Receptor in Xenopus Oocytes

α6/α3β4烟碱型乙酰胆碱受体(α6β4*nAChRs,*代表其他亚基)主要分布于大脑海马区、外周神经节和人源肾上腺嗜铬细胞等,与神经性疼痛和炎症反应、情绪等生理疾病密切相关。本研究拟对大鼠α6/α3β4 nAChR在非洲爪蟾(Xenopus laevis)卵母细胞膜上进行重组表达,并利用双电极电压钳电生理学技术检测该重组受体的功能。在不同浓度的激动剂乙酰胆碱的刺激下,比较了α6/α3和β4亚基的不同配比形成的受体通道开放而产生的电流大小,并用其拮抗剂α−芋螺毒素TxID验证了该受体的敏感性。结果表明,亚基不同配比的α6/α3β4 nAChR均可在非洲爪蟾卵母细胞膜上成功表达,并具有较好的配体门控敏感性。

α6/α3β4 nicotinic acetylcholine receptors(α6β4*nAChRs,the asterisk denotes the possible presence of additional subunits)are mainly distributed in the hippocampus,peripheral ganglia and human adrenal chromaffin cells,and are closely related to neuropathic and inflammatory pain,stress,etc.Recombinant expression of ratα6/α3β4 nAChR on Xenopus laevis oocytes was detected by using the two-electrode voltage clamp to record inward currents elicited by acetylcholine at different concentrations in light ofαandβsubunits at different ratios,and the sensitivity of this receptor was verified by its antagonistα-conotoxin TxID.The results showed thatα6β4 nAChR subtypes with different ratios of subunits were successfully expressed in the oocyte membrane,and had good sensitivity of ligand-gated ion channel function.