谷子丙酮酸磷酸双激酶基因(SiPPDK1)对非生物逆境的响应

Response of Pyruvate Orthophosphate Dikinase(SiPPDK1) to Abiotic Stresses in Foxtail Millet

通过序列比对在谷子基因组中鉴定出一个PPDK基因,命名为Si PPDK1。为揭示该基因对逆境胁迫的响应,本研究通过对Si PPDK1的基因结构、蛋白特征、功能、启动子区域顺势元件、亚细胞定位、进化特征等进行了系统的分析和预测。荧光实时定量PCR检测了该基因在苗期不同逆境、关键生育期干旱以及不同光照条件下的表达。结果表明该基因位于谷子9号染色体,含有17个内含子,有2个可变剪切。功能域分析和多序列比对发现Si PPDK1蛋白具有非常保守的序列结构,并且与其它植物PPDK蛋白非常相似。q RT-PCR表达谱分析表明Si PPDK1基因在苗期被PEG、ABA、Na Cl和低温胁迫强烈诱导。进一步研究表明Si PPDK1基因在拔节、抽穗和灌浆期干旱和不同光照强度条件下均参与了对干旱和光照的胁迫响应,推测该基因参与了谷子对非生物逆境的应答,尤其在抽穗期干旱和光照胁迫应答中起重要作用。

A PPDK gene named Si PPDK1 was identified in foxtail millet genome by sequence alignment.To reveal the response of Si PPDK1 to abiotic stress,the gene structure,protein characteristics,function,cis-elements,subcellular localization predictions and evolution of Si PPDK1 were analyzed and predicted by online tools and software.The quantitative real-time PCR(q RT-PCR)was used to detect the induced expression of Si PPDK1 under different stresses during seedling stage and under drought during key growth stages.The results showed that Si PPDK1 was located on chromosome 9 in the foxtail millet genome,containing 17 introns and 2 alternative transcripts.Functional domain analysis and multiple sequence alignment revealed that the Si PPDK1 protein has a very conserved sequence structure and is very similar to other plant PPDK proteins.q RT-PCR analysis showed that the expression of Si PPDK1 was strongly up-regulated under PEG,ABA,Na Cl and low temperature at its seedling stage.Further studies indicated that Si PPDK1 was involved in drought stress and different light intensity in jointing,heading and filling stage.It is speculated that Si PPDK1 is involved in abiotic stress,especially plays an important role under drought and light stresses at the heading stage.