基于Oncomine和TCGA数据库分析GABRE在结肠癌组织中的表达及生物学意义

Expression and biological significance of GABRE in colon cancer:An analysis based on data mining of Oncomine and TCGA databases

目的:通过挖掘Oncomine和TCGA等数据库信息分析GABRE基因在结肠癌中的表达及其生物学意义。方法:利用Oncomine、TCGA数据库分析GABRE基因在结肠癌组织中的表达及其与患者预后的关系;运用TargetScan、starBase、mirDIP和miRWalk寻找靶向GABRE基因的上游miRNA,并分析其在结肠癌中的表达及其与预后的关系。进一步利用LinkedOmics数据库寻找GABRE共表达基因,并进行GO富集分析及KEGG通路分析。结果:数据库数据分析显示,GABRE在结肠癌组织中高表达且预示着患者预后较差(均P<0.05)。韦恩图显示,hsa-miR-370-3p靶向GABRE,并在正常组织中的表达显著升高(P<0.01)。GABRE基因与OGT、FAM156A基因等表达呈正相关(P<0.05),与ATP5A1、MPDU1基因等表达呈负相关(P<0.05)。GO生物功能及KEGG通路富集分析提示,GABRE基因可能参与蛋白脱烷基化和周期蛋白依赖性蛋白激酶活性调节等生物学过程,并在牛磺酸代谢和NF-κB信号通路等方面富集。结论:GABRE基因在结肠癌患者中高表达且预示着患者预后较差,提示该基因是结肠癌的诊断和治疗的潜在新靶点。

Objective:To explore the expression and biological significance of GABRE gene in colon cancer by mining data in the Oncomine and TCGA databases.Methods:The expression of the GABRE gene in colon cancer tissues and its correlation with the prognosis of patients were analyzed using the Oncomine and TCGA databases.The upstream miRNA targeting GABRE gene was identified using TargetScan,starBase,mirDIP,and miRWalk,and its expression and relationship with prognosis of colon cancer were analyzed.Furthermore,the GABRE co-expression genes were screened using the LinkedOmics database,and the GO enrichment analysis and KEGG pathway analysis were carried out.Results:The results showed that GABRE was highly expressed in colon cancer and indicated a poor prognosis(all P<0.05).The Venn diagram indicated that hsa-miR-370-3p targeted GABRE,and its expression was markedly increased in normal tissues(P<0.01).The expression of GABRE was positively correlated with the expressions of OGT and FAM156A genes,whereas negatively correlated with the expressions of ATP5A1 and MPDU1 genes(all P<0.05).GO biological process function and KEGG pathway enrichment analysis suggested that the GABRE gene may be involved in biological processes including protein dealkylation and regulation of cyclin-dependent protein kinase activity and enriched in taurine metabolism and NF-κB signaling pathway.Conclusions:GABRE gene is highly expressed in patients with colon cancer and indicates a poor prognosis,suggesting that the gene may serve as a potential novel target for the diagnosis and treatment of colon cancer.