欧山种葡萄果实成熟期酚类物质含量及其合成相关基因表达量的比较

Comparison of the content of phenolic compounds and the expression levels of their biosynthesis related genes in ripening stage of Vitis vinifera×Vitis amurensis red grape

用超高效液相色谱法测定6个欧山种红色酿酒葡萄品种(品系)(北红、北玫、北玺、北馨、新北醇、北醇)果实成熟期6种单体酚(芦丁、表儿茶素、白藜芦醇、咖啡酸、没食子酸、儿茶素)含量;实时荧光定量PCR检测单体酚合成相关基因苯丙氨酸裂解酶基因(PAL)、肉桂酸–4–羟化酶基因(C4H)、类黄酮3'–羟化酶基因(F3'H)、类黄酮3',5'–羟化酶基因(F3'5'H)的相对表达量。结果表明:北红葡萄果实中含有较高总酚和单宁含量,分别为13.60和4.69 mg/g,除表儿茶素外,其余5种单体酚含量均较高,儿茶素含量最高,为34.83 mg/kg;北红葡萄的PAL和C4H基因相对表达量较高;除儿茶素和没食子酸外,北红葡萄咖啡酸与F3'5'H基因相对表达量、表儿茶素与F3'H基因相对表达量、芦丁与C4H基因相对表达量呈显著正相关,相关系数均为0.99;北玺葡萄芦丁与C4H基因相对表达量呈显著正相关,相关系数为0.99,与F3'5'H基因相对表达量呈极显著正相关,相关系数为1.00。

Ultra-high performance liquid chromatography(UPLC)method was used to determine the contents of 6 types of monomeric phenols(rutin hydrate,epicatechin,resveratrol,caffeic acid,gallic acid,catechin)from 6 Vitis vinifera×Vitis amurensis red wine grape varieties(strains)(‘Beihong’‘Beimei’‘Beixi’‘Beixin’‘Xinbeichun’‘Beichun’)during fruit ripening.Real-time fluorescent quantitative PCR was used to detect the expression of monomeric phenol synthesis related genes(PAL,C4H,F3'H,F3'5'H).The results showed that Beihong grape fruits contained high total phenols and tannins,which were 13.60 mg/g and 4.69 mg/g respectively;except for epicatechin,the content of the other 5 monomeric phenols in Beihong grape fruit is high,and the catechin content is the highest,which is 34.83 mg/kg.Relative expression levels of PAL and C4H genes in Beihong grapes are relatively high;except for catechin and gallic acid,caffeic acid and F3'5'H gene expression,epicatechin and F3'H gene expression,and rutin and C4H gene expression were significantly positively correlated in Beihong grape,with correlation coefficients both being 0.99.In Beixi grapes,rutin was significantly positively correlated with the relative expression of C4H gene,with a correlation coefficient of 0.99,and was extremely significantly positively correlated with F3'5'H gene expression,with a correlation coefficient of 1.00.