蛹虫草菌丝体蛋白高通量发酵生产技术研究

Study on the High-throughput Fermentation of Cordyceps militaris for Mycelium Protein Production

为了获得高产量的蛹虫草菌丝体蛋白,利用高通量液体发酵技术,在菌丝快速生长阶段,通过连续补料方式调节发酵底物,加快菌丝对物料的持续利用,提高菌丝体的生物量及菌丝体蛋白质含量。试验通过对液体发酵参数的研究得出,当发酵液p H为4.10~4.20,发酵液还原糖含量18 mg·m L-1左右,200 L发酵罐连续补液4次(3.5 L/次,含浓氨水96 m L、葡萄糖1 200 g、酵母膏360 g),发酵69 h,获得的菌丝体生物量为2.360g·100-1m L-1,菌丝体蛋白得率达46.5%,菌丝体蛋白含量1.098 g·100-1m L-1;比普通一段式发酵时间缩短3 h,菌丝体蛋白含量提高68.92%。

In order to obtain high-yield Cordyceps militaris mycelium protein,high-throughput liquid fermentation technology was used to regulate the fermentation substrate through continuous feeding in the rapid growth stage of mycelium,accelerate the utilization of mycelium materials,and improve the biomass and protein matter content of mycelium.According to the study of the parameters of liquid fermentation,when the p H of the fermentation liquid was 4.10~4.20 and the reducing sugar of the corresponding fermentation liquid was about 18 mg·m L-1,nutrients are supplemented for 4 times in 200 L fermentor (3.5 L/time,including 96 m L concentrated ammonia,1 200 g glucose and 360 g yeast extract).After 69 h fermentation,the mycelium biomass was 2.360 g·100-1m L-1,the mycelium protein yield was 46.5%,and the mycelium protein content was 1.098 g·100-1m L-1,compared with the ordinary one-stage fermentation,the fermentation time was shortened by 3 h,and the yield of mycelium protein was increased by 68.92%.