兔D型多杀性巴氏杆菌的分离鉴定及免疫原性研究

Identification and Immunogenic Evaluation of Rabbit Capsular Serotype D Pasteurella multocida

从山东东营发病兔场送检的肝脏病料中分离到1株病原菌,经过菌落形态观察、细菌培养、生化反应、PCR鉴定等,进一步进行了致病性试验和免疫原性试验,结果显示成功分离到1株D型多杀性巴氏杆菌,命名为LD-1,致病性试验表明该菌对兔的致死率为100%,免疫试验结果表明分离菌制成的疫苗攻毒保护率可以达到100%,因此,LD-1有望作为进一步研发D型多杀性巴氏杆菌灭活疫苗的制苗菌株。

A pathogenic bacterium was isolated from the liver of a dead rabbit at Dongying in Shandong.The culturecharacteristics and bacterial stain,biochemistry test and PCR detection of specific genes were examined to charac-terize the isolater.and the pathogenicity and immunogenicity were also conducted.The results showed that a strain ofcapsular serotype D Pasteurella multocida,named LD-1,was successfully isolated.The pathogenicity test showedthat the lethal rate of the strain to rabbits was 100%,and the immune test showed that the protective rate of the vac-cine produced by the strain could reach 100%.the strain LD-1 could be taken as an ideal stain for development ofpotential inactivated vaccine against Pasteurella multocida.