1株南极来源真菌Arthrinium arundinis的次级代谢产物研究

Study on the secondary metabolites of an Antarctic fungus Arthrinium arundinis

目的对1株南极海泥来源真菌Arthrinium arundinis(FNJ20)的次级代谢产物进行研究。方法运用硅胶柱层析、Sephadex LH-20凝胶柱层析和半制备高效液相色谱(HPLC)等方法对A.arundinis(FNJ20)的大米发酵提取物进行分离纯化;通过核磁共振谱(NMR)及质谱(MS)等波谱学方法进行结构解析,通过X射线单晶衍射实验确定化合物的绝对构型,并测定化合物的抗细菌及真菌活性。结果从A.arundinis(FNJ20)的大米发酵提取物中分离鉴定出6个二萜类化合物(1~6)及2个苯并色原酮类化合物(7~8),首次获得化合物1的单晶数据,并确证其绝对构型与文献相反。抗菌活性评价结果表明,化合物8对金黄色葡萄球菌和白色念珠菌具有显著的抑制活性,其MIC值分别为6.25和12.50μmol/L。

Objective To study the secondary metabolites of an Antarctic fungus Arthrinium arundinis(FNJ20).Methods The compounds were isolated by Silica gel and Sephadex LH-20 gel column chromatography,and semi-preparative HPLC from the organic extract of its rice culture.Their structures were identified by comprehensive NMR and MS spectroscopic methods as well as comparison with previously related literature,and the absolute configurations were established by single crystal X-ray diffraction.The antibacterial and antifungal activities of the compounds were determined by microdilution method.Results Six diterpenoids(1~6)and two benzochromones(7~8)were obtained and identified from the crude extract of Arthrinium arundinis(FNJ20).The single crystal diffraction data of compound 1 was obtained for the first time and its absolute configurations were opposite to those of the previously literature.The results of antibacterial and antifungal activities showed that compound 8 exhibited significate inhibitory activity on Staphylococcus aureus and Candida albicans with MIC values of 6.25 and 12.50μmol/L,respectively.