复方血栓通胶囊对高海拔视网膜病变大鼠的保护作用

Protective effect of complex thrombolysis capsule on high altitude retinopathy in rat models

目的:探讨复方血栓通胶囊对高海拔视网膜病变大鼠的保护作用及其作用机制。方法:雄性SD大鼠24只随机分为6组,分别饲养于模拟高海拔5000m的高原舱内2、4、6、10、24、72h,观察各组大鼠视网膜组织形态,HIF-1α的表达和入舱前后大鼠视网膜电图中暗适应最大反应b波振幅的变化;雄性SD大鼠24只随机分为4组,分别予安慰剂,大花红景天口服液,肌苷片及复方血栓通胶囊灌胃喂药7d后,饲养于模拟海拔为5000m模拟舱内10h,观察各组大鼠视网膜组织形态,HIF-1α的表达和入舱前后大鼠视网膜电图中暗适应最大反应b波振幅的变化。结果:高海拔视网膜病变SD大鼠模型中,结果显示随着各实验时间的增加,神经节细胞层细胞肿胀明显,HIF-1α在神经节细胞及内核层细胞胞浆表达增高,均在10h时最为明显。各组SD大鼠进舱自身前后F-ERG暗适应最大反应b波振幅变化分析,4、6、10、72h组F-ERG暗适应最大反应b波振幅显著降低(P<0.05);2h组和4h组(P=0.007)、6h组(P=0.008)、10h(P=0.002)有差异,24h组和4h组(P=0.035)、6h(P=0.040)、10h组(P=0.012)有差异。复方血栓通对SD大鼠高海拔视网膜病变预防作用研究中,结果显示与安慰剂组相比较,复方血栓通组、红景天组及肌苷组的视网膜水肿均明显减轻,但复方血栓通组与红景天组视网膜水肿程度明显低于肌苷组;复方血栓通组及红景天组HIF-1α在神经节细胞层及内核层表达明显下降;各组SD大鼠进舱后F-ERG暗适应最大反应b波振幅比较显示,安慰剂组与复方血栓通组(P=0.032),红景天组(P=0.001)均有差异。结论:在模拟高海拔环境下,复方血栓通可能是通过抑制HIF-1α的表达对大鼠高海拔视网膜病变有保护作用,但是具体作用机制有待我们进一步研究。

AIM:To investigate the protective effect of complex thrombolysis capsule on high altitude retinopathy in rat models and its possible mechanism.METHODS:Twenty-four adult male SD rats were randomly divided into 6 groups and were put into plateau environment simulation experimental chamber at simulated altitude of 5000m for 2,4,6,10,24,72h respectively.Retinal pathology,HIF-1αand the b wave amplitudes of Max-R of flash ERG were examined by HE,IHC and flash ERG.Twenty-four adult male SD rats were randomly divided into 4 groups and were given respectively placebo,rhodiolarosea,inosine tablet and compound xueshuantong capsule by gavage for 7d.They were put into plateau environment simulation experimental chamber at simulated altitude of 5000m for 10h.Retinal pathology,HIF-1αand the b wave amplitudes of Max-R of flash ERG were examined by HE,IHC and flash ERG.RESULTS:In the SD rat model of high-altitude retinopathy,with the increase of experimental time,the ganglion cell layer of rat’s retina showed obvious edema and HIF-1αexpression increased in the cytoplasm of ganglion cells and core cells.All of them were most obvious at 10h.Compared with the self-comparison of b wave amplitudes of Max-R of flash ERG in each group of SD rats before and after entering in plateau environment simulation experimental chamber,the b wave amplitudes of Max-R in 4h,6h,10h and 72h were dramatically decreased(P﹤0.05).And the 2h,4h(P=0.007),6h(P=0.008),10h(P=0.002)were statistically significant differences,the 24h and 4h(P=0.035),the 6h(P=0.040)and 10h(P=0.012)were also statistically significant differences.In the study of protective effect of complex thrombolysis caps on high altitude retinopathy in rat models,the results showed that the rat retinal edema of rhodiolarosea group,inosine tablet group and compound thrombosis capsule group and HIF-1αexpression in ganglion cell layer of compound thrombosis group and rhodiolarosea group were significantly reduced comparing with the placebo group.Test for homogeneity of variance and one-way ANOVA were used to test the difference of b wave amplitudes of Max-R of flash ERG in four groups of SD rats after entering in the plateau environment simulation experimental chamber,the results showed the complex thrombolysis caps group(P=0.032)and rhodiola rose group(P=0.001)was significantly lower than placebo group.CONCLUSION:Compound thrombosis caps may have a protective effect on highaltitude retinopathy in rats by inhibiting the expression of HIF-1α,however,the specific mechanism needs to be further studied.