基于生物信息学检测TRIM47基因在肝癌中的表达及意义

Expression of TRIM47 and its significance in liver cancer base on bioinformatic test

目的探讨三结构域蛋白47(TRIM47)基因在肝癌中的表达及其临床意义。方法通过生物信息学方法,利用UALCAN、HPA数据库分析TRIM47在肝癌中的基因表达和蛋白表达。利用Kaplan-Meier生存数据库分析肝癌患者总生存率,评估TRIM47在肝癌患者预后中的价值。利用cBioPortal数据库分析肝癌患者中TRIM47的基因突变频率,以及TRIM47基因突变对肝癌患者总生存率的影响。构建稳定表达TRIM47的肝癌细胞株Hep3B、Huh-7,采用qRT-PCR检测肝癌细胞TRIM47 mRNA,CCK-8法检测其细胞增殖能力。肝癌TRIM47 mRNA相对表达量和增殖能力比较采用秩和检验或t检验。结果UALCAN数据库分析显示肝癌组织中TRIM47 mRNA相对表达量中位数为18.95(10.79,31.81),明显高于正常肝组织中的7.09(5.76,9.61)(P<0.05)。HPA数据库分析显示肝癌组织中TRIM47蛋白水平呈高表达,而在正常肝组织中呈低表达。UALCAN数据库分析显示随着肿瘤组织中TRIM47表达水平的升高,患者临床分期、肿瘤分级越差(P<0.05)。Kaplan-Meier生存数据库分析显示低表达TRIM47的肝癌患者总体生存期更长(HR=1.59,95%CI:1.12~2.26;P<0.05)。cBioPortal数据库分析显示TRIM47在肝癌患者中的基因突变率可达14%,且能明显影响患者总生存率(P=0.0136)。Hep3B-TRIM47和Huh-7-TRIM47肝癌细胞TRIM47 mRNA平均相对表达量分别为(4.87±0.14)×10^-3、(2.47±0.47)×10^-3,明显高于对照组的(0.60±0.08)×10^-3、(0.18±0.05)×10^-3(t=26.560,4.856;P<0.05)。Hep3B-TRIM47和Huh-7-TRIM47肝癌细胞株增殖能力显著增强。结论TRIM47基因可通过促进肝癌细胞增殖影响肝癌患者的预后,TRIM47可能成为预测肝癌患者预后的一种新型标记物。

Objective To investigate the expression and clinical significance of tripartite motifcontaining 47(TRIM-47)gene in liver cancer.Methods The expression of TRIM47 mRNA and protein in liver cancer were analyzed by bioinformatics methods using UALCAN and HPA databases.The overall survival of liver cancer patients was analyzed by Kaplan-Meier survival database.The prognostic value of TRIM47 in liver cancer patients was evaluated.The frequency of TRIM47 gene mutation was analyzed by cBioPortal database.The effect of TRIM47 gene mutation on the overall survival of liver cancer patients was assessed.The liver cancer cell lines Hep3B and Huh-7 which stably expressed TRIM47 were constructed.The expression of TRIM47 mRNA in liver cancer cells was detected by qRT-PCR.The cell proliferation ability was detected by CCK-8 assay.The relative expression of TRIM47 mRNA and the proliferation ability of liver cancer cells were compared by rank-sum test or t test.Results UALCAN database analysis showed that the median relative expression of TRIM47 mRNA in liver cancer tissues was 18.95(10.79,31.81),significantly higher than 7.09(5.76,9.61)in normal liver tissues(P<0.05).HPA database analysis demonstrated that TRIM47 protein highly expressed in liver cancer tissues,whereas lowly expressed in normal liver tissues.UALCAN database revealed that with the up-regulation of TRIM47 expression in tumor tissues,the clinical stage and tumor grade became significantly worse(both P<0.05).Kaplan-Meier survival database analysis showed that the overall survival of liver cancer patients with low expression of TRIM47 was significantly longer(HR=1.59,95%CI:1.12-2.26;P<0.05).cBioPortal database analysis indicated that the mutation rate of TRIM47 gene in patients with liver cancer reached up to 14%,and significantly affected the overall survival(P=0.0136).The mean relative expression levels of TRIM47 mRNA in Hep3B-TRIM47 and Huh-7-TRIM47 liver cancer cells were(4.87±0.14)×10^-3 and(2.47±0.47)×10^-3,significantly higher than(0.60±0.08)×10^-3 and(0.18±0.05)×10^-3 in the control group(t=26.560,4.856;P<0.05).The proliferation ability of Hep3B-TRIM47 and Huh-7-TRIM47 cell lines was significantly enhanced.Conclusions TRIM47 can affect the clinical prognosis of patients with liver cancer by promoting the proliferation of liver cancer cells.TRIM47 may become a novel biomarker for predicting the prognosis of liver cancer patients.