不同温度下恩诺沙星在克氏原螯虾体内的药代动力学

Pharmacokinetics of enrofloxacin in Procambarus clarkii at different temperatures

在18℃和25℃2种温度下,对克氏原螯虾注射剂量为20μg/kg的恩诺沙星药液,分析恩诺沙星和环丙沙星在克氏原螯虾体内的药物动力学特征和残留规律。结果显示,恩诺沙星及环丙沙星在血淋巴、肌肉、肝胰腺中回收率80%~110%,检测值日内变异系数均小于5%,日间变异系数均小于6%,检测限均在0.01μg/mL(μg/g)以下。18℃时恩诺沙星在克氏原螯虾血淋巴、肌肉、肝胰腺中的T max分别为0.0830、0.3447、1.9335 h,25℃时恩诺沙星在克氏原螯虾血淋巴、肌肉、肝胰腺中的T max分别为0.0830、0.2634、1.1658 h,温度提高加快了克氏原螯虾中恩诺沙星的吸收。消除半衰期T 1/2β大小顺序为肝胰腺(55.7403 h)>肌肉(52.7437 h)>血浆(18.6087 h)。以恩诺沙星和环丙沙星作为残留标志物,25℃时恩诺沙星在肝胰腺的消除期为311.47 h,18℃时恩诺沙星在肝胰腺的消除期为417.77 h,根据本试验结果,以国家标准0.2μg/g为残留标准,建议恩诺沙星休药期为325(℃·d)。

In this experiment,at 18℃and 2℃,enrofloxacin was injected at a dose of 20μg/kg to study the pharmacokinetics characteristics and residues of enrofloxacin and ciprofloxacin in Procambarus clarkii.The results showed that the recovery rates of enrofloxacin and ciprofloxacin in the hemolymph,muscle,hepatopancreas and pancreas were from 80%to 110%.The intra-day variation coefficients of the detection values were less than 5%,and the inter-day variation coefficients were less than 6%.The detection limits were all below 0.01μg/mL(μg/g).The T max of enrofloxacin in the hemolymph,muscle,hepatopancreas at 18℃was 0.0830 h,0.3447 h,and 1.9335 h,respectively,and the T max of ciprofloxacin was 0.0830 h,0.2634 h,and 1.1658 h,respectively.Increasing the temperature accelerated the absorption of enrofloxacin in P.clacherii.The order of elimination half-life T 1/2βwas hepatopancreas(55.7403 h)>muscle(52.7437 h)>plasma(18.6087 h).With enrofloxacin and ciprofloxacin as residual markers,the elimination period of enrofloxacin in the hepatopancreas and pancreas was 311.47 h at 25℃,and the elimination period of enrofloxacin in hepatopancreas and pancreas was 417.77 h at 18℃.It is recommended that the enrofloxacin withdrawal period is 325(℃·d).