五溴联苯醚(BDE-99)和羟基五溴联苯醚(5-OH-BDE-99)经由THRβ影响斑马鱼胚胎眼部色素的沉着

BDE-99 and 5-OH-BDE-99 Affect the Pigmentation of the Eyes of Zebrafish Embryos via THRβ

多溴联苯醚及其羟基代谢物(OH-BDEs)被认为是内分泌干扰物,特别是对甲状腺激素调节的影响。调查并比较了BDE-99及5-OH-BDE-99对甲状腺素受体的影响。斑马鱼胚胎暴露于不同浓度的BDE-99和5-OH-BDE-99中,导致斑马鱼眼球黑色素的色素沉着减少,使用原位杂交以及定量PCR方法检测,发现THRβ在斑马鱼前脑部位表达,并有明显的降低。通过体外实验也证实BDE-99和5-OH-BDE-99能够与THRβ受体结合,尤其是5-OH-BDE-99的结合更为强烈。研究表明,BDE-99和5-OH-BDE-99对斑马鱼胚胎早期THRβ的活性有干扰作用,并导致眼部黑色素沉积减少。上述研究结果对BDE-99和5-OH-BDE-99的生物毒理学机制研究有一定的借鉴意义。

Polybrominated diphenyl ethers and their hydroxyl metabolites(OH-BDEs)are considered to be endocrine disruptors.They are found in human serum and particularly involved in the regulation of thyroid hormones.The effects of BDE-99 and 5-OH-BDE-99 on the thyroxine receptors were investigated and compared in this study.Zebrafish embryos were exposed to different concentrations of BDE-99 and 5-OH-BDE-99.The results showed that exposure to BDE-99 and 5-OH-BDE-99 resulted in reduced pigmentation in the eyes of zebrafish embryos.In situ hybridization and quantitative PCR analyses revealed that THRβwas expressed in the forebrain and its transcriptional expression was significantly suppressed by the two chemicals.In addition,in vitro experiments demonstrated that BDE-99 and 5-OH-BDE-99 successfully bound to the THRβreceptor.This study indicates that BDE-99 and 5-OH-BDE-99 can disturb the thyroid endocrine system by altering the the expression of THRβin zebrafish.This study helps better understand the mechanisms of the toxicity of BDE-99 and 5-OH-BDE-99 in zebrafish.