微电流对畜禽养殖污水中常见细菌的杀灭效果研究

Killing Effect of Microcurrent against Commonly Observed Bacteria in Livestock and Poultry Sewage

[目的]观察微电流对水体中细菌的杀灭效果,为养殖业污水消毒寻找新方法提供理论支撑。[方法]通过开展悬液定量杀菌试验、影响因素测定试验和模拟现场试验,对微电流杀灭大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌和枯草芽孢杆菌的效果进行研究,探讨温度和pH值对消毒效果的影响,并以人工湖水和养殖场污水为消毒对象进行模拟现场试验。[结果]微电流消毒作用4 min时,对4种菌悬液的杀灭对数值均>5.0。温度和pH值对微电流消毒效果无影响。利用微电流对人工湖水和养殖场污水进行消毒,作用4 min时杀菌率达到99.99%,水中细菌基本已被杀灭;作用5 min时,水中细菌菌落总数下降至0 CFU/mL,能达到完全杀灭细菌的效果。[结论]微电流对畜禽养殖污水中常见细菌具有杀灭作用,在畜禽养殖业消毒方面具有广阔的应用前景。

[Objective]To evaluate the killing effect of microcurrent against bacteria in water body,and to provide basis for developing new disinfection techniques for livestock and poultry sewage.[Method]Suspension quantitative bactericidal test,influencing factors determination test and simulated field test were conducted to assess the killing effect of microcurrent against Escherichia coli(E.coli),Staphylococcus aureus(S.aureus),Pseudomonas aeruginosa(P.aeruginosa)and Bacillus subtilis(B.subtilis),to evaluate the impact of temperature and pH value on disinfection effect,and to investigate the disinfection effect of microcurrent on artificial lake water and waste water from livestock and poultry farms.[Result]The killing log values against the above 4 bacterial suspension were all higher than 5.0 after the treatment of microcurrent for 4 min.Temperature and pH value had no impacts on disinfection effect of microcurrent.After the treatment of microcurrent for 4 min,99.99%of the total bacteria in the artificial lake water and waste water from livestock and poultry farms were killed,indicating that the bacteria in the water samples were almost killed.When treatment duration extended to 5 min,the total number of bacterial colonies in the water samples were reduced to 0 CFU/mL,indicating that the bacteria in the water samples were completely killed.[Conclusion]Microcurrent has killing effect against the commonly observed bacteria in livestock and poultry sewage,and has broad application prospect in disinfection in livestock and poultry rearing industry.