仙鹤草不同药用部位HPLC指纹图谱及化学模式识别研究

The Establishment of HPLC Fingerprint and Chemical Pattern Recognition of Agrimonia pilosa Ledeb.in Different Medicinal Parts

目的为仙鹤草药材的质量评价提供方法。方法采用高效液相色谱法,建立仙鹤草不同药用部位指纹图谱,进行相似度评价、聚类分析和主成分分析。结果16批仙鹤草地上部分、茎、叶各标定了20、16、14个共有峰,相似度均大于0.89;大致可聚为三类,表明地上部分与茎化学成分接近,与叶化学成分差异较大。经主成分及方差分析可知仙鹤草叶为色谱峰2、3、4、5、11、13、14(槲皮苷)、15、16、18、19的富集部位,茎为色谱峰1、7、8、9、10(鞣花酸)、12、17、20的富集部位。结论仙鹤草不同药用部位HPLC指纹图谱存在差异,可用于评价仙鹤草各药用部位药材的质量,为进一步探讨各药用部位药理作用及药材资源的合理利用提供数据支撑。

Objective To provide the quality evaluation of Agrimonia pilosa Ledeb..Methods To establish the fingerprint of Agrimonia pilosa Ledeb.by HPLC,and to study the similarity evaluation,cluster analysis and principal component analysis of different medicinal parts.Results There are 20 common peaks in the aboveground part,16 common peaks in the stem and 14 common peaks in the leaf;the similarity is 0.89 above.Cluster analysis can be divided to three categories,which showed components of the aboveground part are similar to stem,but different from leaf.Principal component analysis and variance analysis show the leaves of crane grass are the enrichment sites of chromatographic peaks 2,3,4,5,11,13,14(quercetin),15,16,18,19,the stems are the enrichment sites of chromatographic peaks 1,7,8,9,10(ellagic acid),12,17,20.Conclusion HPLC fingerprint of Agrimonia pilosa Ledeb.in different medicinal parts are different,which can be used to evaluate the quality of different parts of Agrimonia pilosula,and provide data support for further study of the pharmacological effects of different parts and the rational utilization of medicinal resources.