Kininogen 1在非小细胞肺癌体液和组织中的表达及临床意义

Clinical significance of kininogen 1 expression in non-small cell lung cancer biofluids and tissues

目的通过检测非小细胞肺癌患者血浆、肺泡灌洗液(bronchoalveolar lavage fluid,BALF)、尿液及组织中激肽原1(kininogen 1,KNG1)的表达,了解其在非小细胞肺癌中的临床意义。方法收集40例非小细胞肺癌患者和20例肺良性疾病对照者的血浆、BALF和尿液标本,通过酶联免疫方法检测血浆、BALF和尿液中KNG1的含量,应用免疫组织化学法检测40例非小细胞肺癌及其癌旁对照标本KNG1的表达。结果非小细胞肺癌患者血浆、BALF和尿液中KNG1浓度显著高于对照组(P均<0.001)。受试者工作特征(receiver operating characteristic,ROC)曲线分析显示KNG1在血浆、肺泡灌洗液和尿液中的曲线下面积分别为0.79、0.89和0.87,差异均有统计学意义(P<0.05),各标本的KNG1均具有一定诊断非小细胞肺癌能力,诊断界值分别为血浆为1653.6 mg/L,BALF为52.1 mg/L,尿液为2.3 mg/L,尿液的一致性最高(Kappa=0.59),尿液诊断效果优于血浆和BALF。在40例非小细胞肺癌和邻近正常组织中的免疫组织化学染色结果显示非小细胞肺癌组织中KNG1表达高于癌旁正常组织。结论KNG1在非小细胞肺癌患者的血浆、肺泡灌洗液、尿液和组织中是高表达的,在体液诊断尤其是尿液中具有一定诊断非小细胞肺癌的能力。

Objective To evaluate the clinical significance in the diagnosis and prognosis of non-small cell lung cancer(NSCLC)by detecting the expression of kininogen-1(KNG1)in plasma,alveolar lavage fluid,urine and tissues of patients with NSCLC.Methods The plasma,alveolar lavage fluid and urine samples were collected from 40 patients with NSCLC and 20 patients with benign lung disease(as control group).Enzyme-linked immunosorbent assay was used to detect the content of KNG1 in plasma,bronchoalveolar lavage fluid(BALF)and urine.The expression of KNG1 in 40 NSCLC and its paracancerous control samples was detected by immunohistochemistry.Results The levels of KNG1 in plasma,BALF and urine of patients with NSCLC were significantly higher than those of the control group.Receiver operating characteristic(ROC)curve analysis showed that KNG1 had an area under curve(AUC)of 0.79,0.89 and 0.87 in plasma,BALF,and urine respectively.All had statistical significance(P<0.05),indicating that KNG1 of each specimen had certain ability to diagnose NSCLC.The diagnostic cut-off values were 1653.6 mg/L for plasma,52.1 mg/L for BALF and 2.3 mg/L for urine.The results showed that the consistency of urine was the highest(Kappa=0.59),indicating that the diagnostic effect was better than plasma and BALF.Immunohistochemical staining of 40 NSCLC and adjacent normal tissues showed that KNG1 was higher in NSCLC tissues than in adjacent normal tissues.Conclusions KNG1 was highly expressed in plasma,alveolar lavage fluid,urine and tissues of NSCLC,and had a certain ability to diagnose NSCLC in body fluids,especially urine.