基于多元统计分析的不同诃子属药材多指标成分研究

Multi-indicative component ananlysis of different species of Terminalia L.based on multivariate statistical analysis

建立了诃子属药材的多指标成分含量测定方法,为诃子属药材的质量评价提供依据。测定44批不同诃子属药材中7个鞣质类成分含量,并结合方差分析、聚类分析(HCA)、主成分分析(PCA)和正交偏最小二乘法-判别分析(OPLS-DA)评价诃子属3种不同药材的鞣质类成分差异。可见诃子属3种药材中7个成分含量存在明显差异,方差分析结果显示,不同品种间的成分含量差异均具有统计学意义(P<0.05);聚类分析、主成分分析及正交偏最小二乘法-判别分析结果显示,不同品种药材间7个化学成分含量上存在一定差异,主成分分析及正交偏最小二乘法-判别分析分析结果确定不同诃子属药材间的主要差异成分为安石榴苷A、安石榴苷B、诃子酸、柯里拉京、诃藜勒酸、没食子酸。本研究建立的方法简便、准确,为诃子属药材的质量控制提供了可靠的分析方法与参考依据。

To establish a method for content determination of multi-indicative components of Terminalia L.and provide a reference for the quality evaluation of Terminalia L..The content of 7 tannins were measured in 44 batches of different species of Terminalia L.and the analysis were conducted by variance analysis,cluster analysis(HCA),principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)to evaluate the differences in the tannis content among the three different species of Terminalia L..The results showed that there were distinct differences among 7 tannins content among the three species of Terminalia L..Variance analysis demonstrated that the differences of content were statistically significant(P<0.05).HCA,PCA and OPLS-DA suggested the differences among the species and the results analyzed by PCA and OPLS-DA indicated that the main different components were punicalagin A,punicalagin B,chebulinic acid,corilagin,chebulagic acid and gallic acid.The method is convenient and accurate,which provides a reliable analytical method and reference for the quality control of Terminalia L..