青藏高原黑果枸杞花青素对人肝癌细胞增殖和自噬的影响

Effect of anthocyanidin of Lycium ruthenicum Murry from Qinghai Tibet Plateaun on the proliferation and autophagy of HepG2 cells

探究黑果枸杞花青素在体外对人肝癌HepG2细胞增殖和自噬的影响。利用CCK-8法测定细胞活力,EdU和细胞划痕试验检测细胞增殖和迁移效果,RT-PCR和Western blot检测增殖和自噬相关基因的mRNA和蛋白表达。结果显示,黑果枸杞花青素可有效抑制人肝癌HepG2细胞的增殖和迁移;上调增殖因子(LATS1、LATS2和MOB1)和自噬因子(Beclin-1、LC3-Ⅱ和AMPK),并下调增殖因子YAP的mRNA水平;下调自噬因子p-mTOR和细胞周期因子CDK4,并上调自噬因子p-AMPK和LC3-Ⅱ的蛋白表达。由此推测黑果枸杞花青素可在体外抑制人肝癌HepG2细胞增殖和迁移,并促进人肝癌HepG2细胞发生自噬。

To explore the effect of Lycium ruthenicum Murray anthocyanidin on proliferation and autophagy of HepG2 cells in vitro,the viability of HepG2 cells were detected by CCK-8.Besides,HepG2 cells proliferation and migration were explored by EdU and cell scratch test.Then RT-PCR and Western blot were performed to explore the expression of proliferation-related genes and autophagy-related genes at mRNA and protein level,respectively.We found that L.ruthenicum anthocyanidin could significantly inhibit the proliferation and migration of HepG2 cells.The mRNA expression of proliferation factors(LATS1,LATS2 and MOB1)and autophagy factors(Beclin-1,LC3-Ⅱand AMPK)induced by L.ruthenicum anthocyanidin were up-regulated,while the mRNA expression of proliferation factor YAP induced by L.ruthenicum anthocyanidin were down-regulated.Furthermore,L.ruthenicum anthocyanidin decreased autophagy factor p-mTOR and cyclin factor CDK4 protein expression,while enhanced autophagy factors(p-AMPK and LC3-Ⅱ)protein expression.In conclusion,L.ruthenicum anthocyanidin can inhibit the proliferation and migration,and promote the autophagy of HepG2 cells in vitro.