阿魏酸钠对增生性瘢痕成纤维细胞增殖及胶原合成的影响

Effects of sodium ferulate on proliferation and collagen synthesis of human hypertrophic scar fibroblasts

探讨阿魏酸钠(SF)对增生性瘢痕成纤维细胞(HSFb)增殖及胶原合成的影响。体外培养HSFb,MTT法计算SF的LC50及最佳药物时间后,分为空白对照组、SF干预组(高、中、低浓度分别为0.3、0.03、0.003 mg/mL),培养72 h后,在倒置显微镜和透射电镜下观察HSFb微观形态学变化;MTT法、Western blot法检测SF对HSFb细胞增殖和I、Ⅲ胶原蛋白表达的影响。结果显示,与空白对照组相比,倒置显微镜下HSFb细胞明显减少,电镜下见核固缩,线粒体轻度肿胀,粗面内质网扩张呈囊泡状,有较多空泡、自噬的产生;MTT法显示各浓度SF干预组对HSFb增殖均有明显抑制作用(P<0.01);Western blot法检测显示低浓度SF对I胶原蛋白表达有一定的抑制作用(P<0.05),高、中浓度SF能明显抑制HSFb的I、Ⅲ胶原蛋白的表达(P<0.01)。本研究结果表明,SF能改变HSFb的微观形态,抑制HSFb的细胞增殖及Ⅰ、Ⅲ型胶原蛋白的表达。

The study attempts to investigate the effects of sodium ferulate(SF)on the proliferation and collagen synthesis of the cultured human hypertrophic scar fibroblast(HSFb).After HSFb cells were cultured in vitro and SF LC50 and optimal drug time were calculated with MTT method,the cells were divided into blank control group and SF intervention groups(high,medium and low concentration rates were 0.3,0.03,0.003 mg/mL,respectively).After HSFbs were cultured for 72 hours,they were observed under inverted microscope and transmission electron microscope to detect their morphological changes.The MTT test and Western blot method were used to detect the effects of SF on the cell proliferation of HSFb and the expression of collagen cells I and III.The results showed that,compared with the blank control group,the HSFb cells were significantly reduced under inverted microscope.Under the electron microscope,the results showed that the nuclei were contracted,that the mitochondria were slightly swollen,that the rough endoplasmic reticulum was expanded into vesicles,and that there were more vacuoles and autophagy.With MTT test,the results showed that SF in each concentration group can significantly inhibit the proliferation of HSFb(P<0.01);With Western blot method,the results showed that SF in high and medium concentration groups can significantly inhibit the expression collagen cells I andⅢ(P<0.01)of HSFb and SF in low concentration group could inhibit the expression of collagen cells I to some extent(P<0.05).The results of this study indicate that SF can transform the micro morphology of HSFb and inhibit the cell proliferation of HSFb and the expression of collagen cells of I and III.