PEG-6000模拟水分胁迫对狗枣猕猴桃幼苗生理特性的影响

Effects of PEG-6000 Simulated Water Stress on Physiological Properties of Actinidia kolomikta Seedlings

为狗枣猕猴桃的引种训化提供理论依据,以蒸馏水作对照(CK),用PEG-6000(聚乙二醇)浓度为5%、10%、15%、20%、25%、30%水溶液进行狗枣猕猴桃模拟水分胁迫试验,于胁迫处理72 h后测定叶片丙二醛(MDA)含量、游离脯氨酸含量、过氧化物酶(POD)活性和超氧化物歧化酶(SOD)活性,研究干旱条件下狗枣猕猴桃幼苗的生理特性,探讨其对干旱的抗性。结果表明:SOD、POD活性和游离脯氨酸、丙二醛含量随PEG-6000浓度的增加而增加,当浓度≥25%时,各项指标增加明显,狗枣猕猴桃幼苗可通过提高体内渗透调节物质含量和抗氧化酶活性以降低干旱环境对自身的损害。

To provide a theoretical reference for the introduction and training of Actinidia kolomikta,taking distilled water as the control(CK),a simulated water stress experiment was conducted on A.kolomikta with various concentrations of PEG-6000 aqueous solution(5%,10%,15%,20%,25%and 30%),after 72 hours of stress treatment,the leaf malondialdehyde(MDA)content,free proline content,peroxidase(POD)activity,and superoxide dismutase(SOD)activity were measured to study the physiological characteristics of A.kolomikta seedlings,so as to explore the resistance to drought.Results:SOD activity,POD activity,free proline,and malondialdehyde content increased with PEG-6000 concentration,and each index increased significantly when PEG-6000 concentration was≥25%A.kolomikta seedlings can reduce the drought damage by increasing the content of osmotic adjustment substances and antioxidant enzyme activity.