胡黄连苷Ⅱ苷元的制备及其对缺血再灌注损伤小鼠治疗作用的研究

Preparation of the Picroside Ⅱ Aglycone and Its Therapeutic Effect on Ischemia Reperfusion Injury in Mice

利用β-葡萄糖苷酶对胡黄连苷Ⅱ(1)进行酶解,得到一种新的环烯醚萜类化合物,通过理化性质、纯度检验及质谱、1H NMR、13C NMR等鉴定,确定为一种新的化合物,即胡黄连苷Ⅱ苷元,命名为Y012(2)。药效学试验结果表明,2对脑缺血再灌注损伤具有很好的治疗作用,可以有效降低大鼠行为学评分及脑梗死面积比,并且可显著降低各炎症因子水平。

In this study,tricopteridⅡ(1)was hydrolyzed in the catalysis of β-glucosidase,and a new iridoid compound[named Y012(2)]was obtained,which was identified as tricopteridⅡaglycon by physical and chemical property inspection,mass spectrometry,1H NMR,and 13C NMR.The pharmacodynamic test showed that 2 had a good therapeutic effect on cerebral ischemia-reperfusion injury,and it could effectively reduce rat behavior score and cerebral infarction area ratio.In addition,it could also significantly reduce the level of inflammatory factors.