复方金栀制剂对脂多糖致热鸡解热作用

Studies on Antipyretic Effect of Compound Jinzhi Preparation on Lipopolysaccharide-induced Pyrexia in Chickens

【目的】复方金栀制剂由具有清热解毒功效的青蒿、金银花和栀子组方,研究其对禽类炎症引起发热的解热效果。为禽类中药解热药的筛选提供药效学依据。【方法】用腹腔注射脂多糖的方法制备鸡只炎症发热模型,肌肉注射260 mg/100g体重复方金栀制剂,注射前后每隔1 h监测鸡只肛门体温。【结果】脂多糖注射后2 h发热模型组鸡只体温极显著高于空白对照组(P<0.01),且持续到注射后7 h。发热鸡只复方金栀制剂给药后1 h体温显著低于发热模型组(P<0.05),之后体温同发热模型组比较差异不显著(P>0.05)。不同给药处理与时间交互对鸡只体温影响均极显著(P<0.01)。【结论】复方金栀制剂对脂多糖引起的鸡只发热具有解热作用,但作用时间较短。

【Objective】The compound Jinzhi preparation(CJZ)is composed of Artemisiae annuae herba,Lonicera japonica flos,and Gardenia jasminoides ellis,which were found to have antipyretic and detoxifying functions.The present study aims to investigate the antipyretic effect of CJZ induced by inflammation in avian.【Methods】Building pyretic model of chicken inflammation by intraperitoneal injection of lipopolysaccharide(LPS),followed by intramuscular injection of CJZ in a dose of 260 mg/100g body weight.The anal temperature of chickens was monitored per hour before and after injection.【Results】The present results showed that anal temperature in the pyretic model group was significantly higher than that of the blank control group at 2 h after LPS injection(P<0.01),and continued to 7 h after the injection.Anal temperature in the Jinzhi group was significantly lower than that in the pyretic model group at 1 hour after administration of JCZ(P<0.05),however,it was not significantly different from that in the pyretic model group from 2 h to 6 h after administration(P>0.05).Two-way ANOVA results showed that the effects of different drug administration and time interactions on chicken’s temperature were significantly different(P<0.01).【Conclusion】The above results demonstrated that CJZ has antipyretic effect on LPS-induced pyrexia in chickens,but the action time is short.These results can be used as reference for the pharmacodynamic studies on antipyretic drugs in avian.