响应面法优化微波提取枇杷花槲皮素工艺及其对酒精分解关键酶活性的影响

Optimization of Microwave-assisted Extraction of Quercetin from Loquat Flowers by Response Surface Methodology and the Effects on Activity of Alcoholic Intoxication Enzyme

以阴干的枇杷花及花蕾为原料,采用60%乙醇为溶剂,微波辅助提取其中的槲皮素,研究料液比、微波时间、微波功率对枇杷花槲皮素提取量的影响,采用响应面法Box-Behnken组合设计三因素三水平优化槲皮素提取条件。结果表明,最佳提取条件是料液比1∶15 g/mL、微波提取时间25 min、微波提取功率600 W;在此条件下提取量为8.26 mg/g。将枇杷花槲皮素使用HP-20型树脂柱层析纯化后纯度为64.87%。该纯化品红外光谱扫描分析结果表明:在1663、1611、1382 cm-1处有槲皮素特征峰。探讨枇杷花槲皮素对酒精分解过程中乙醇脱氢酶(ADH)、乙醛脱氢酶(ALDH)、过氧化氢酶(CAT)活性的影响,结果表明酶活力均有一定的提高,激活率为24.83%、29.18%、22.36%。枇杷花槲皮素对酒精分解过程中关键酶均有一定的激活作用,为开发天然产物解酒护肝辅助食品提供一定理论基础。

Quercetin was extracted from loquat flower dried in shade by microwave-assisted extraction with 60%ethanol as solvent.The effects of material-liquid ratio,microwave time and microwave power on quercetin extraction from loquat flower were studied.The Box-Behnken response surface method was used to optimize the extraction process by three factors and three levels.The results indicated that the optimum extraction conditions were solid-liquid ratio of 1∶15(g/mL),microwave time of 25 min and microwave extraction power of 600 W;the extraction amount of quercetin from loquat flower was 8.26 mg/g.Quercetin from loquat flower was purified by HP-20 column chromatography,the purity of quercetin obtained was 64.87%.Infrared spectrum scans indicated that quercetin characteristic peaks existed at 1663,1611 and 1382 cm-1.The effects of quercetin from loquat flower on the activities of ADH,ALDH and CAT in the process of alcoholic intoxication were investigated.The results for the test showed that the enzyme activities were all improved to a certain extent and the activation rates were 24.83%,29.18%,22.36%.Quercetin from loquat flower has certain activation effect on key enzymes in alcohol intoxication process,which provides certain theoretical basis for the development of natural product anti-alcoholism and food of auxiliary liver protection.