VpSBP3基因负向调控转基因拟南芥盐胁迫抗性

VpSBP3 Gene Negatively Regulates Salt StressResistance in Transgenic Arabidopsis thaliana

葡萄作为世界上重要的落叶果树,具有较高的经济、生态以及社会效益。近年来,葡萄受到盐胁迫的影响,导致其产量与品质都有所降低。本研究利用同源克隆法获得VpSBP3转录因子,并利用花絮浸染法将其转入拟南芥中获得转基因株系。在盐胁迫下,测定了野生对照、pCAMBIA2300空载体对照和VpSBP3转基因拟南芥株系的萌发率、根长、电导率、MDA含量等指标。结果发现在盐胁迫条件下,相较于对照,转基因株系的种子萌发率更低、根长更短、相对电导率和丙二醛的含量更高。表明该转录因子可能在转基因拟南芥盐胁迫响应中具有负向调控作用,这为以后SBP家族基因在葡萄抗逆性方面的研究奠定了前期理论基础。

As an important deciduous fruit tree in the world,grape has high economic,ecological and social benefits.In recent years,grape is affected by salt stress,which leads to the decrease of its yield and quality.In this study,VpSBP3 transcription factors were obtained by homologous cloning and transferred into Arabidopsis thaliana to obtain transgenic lines.Under salt stress,the germination rate,root length,electrical conductivity and MDA content of wild control,pCAMBIA2300 empty vector control and VpSBP3 transgenic Arabidopsis thaliana lines were determined.The results showed that under salt stress,compared with the control,the transgenic lines had lower seed germination rate,shorter root length,higher relative electrical conductivity and higher MDA content.It is suggested that the transcription factor may have a negative regulatory effect on the salt stress response of transgenic Arabidopsis thaliana.This laid a theoretical foundation for the future study of SBP family genes in grape stress resistance.