黑木耳发酵产物对HepG2细胞脂质代谢及糖代谢的影响

Fermentation Products of Auricularia auricular on Lipid Metabolism and Glucose Metabolism in HepG2 Cells

目的:通过建立体外HepG2细胞脂肪累积模型及正常HepG2细胞模型评价不同分子质量黑木耳发酵产物对HepG2细胞脂质代谢及糖代谢的影响,揭示不同分子质量黑木耳发酵产物对细胞脂质代谢及糖代谢的调节作用及差异。方法:采用超滤法将黑木耳发酵上清液分为0~10,10~50,50~100,100~300 ku,以及>300 ku的分子质量,并通过恒温干燥获得黑木耳发酵产物。通过HepG2细胞脂肪累积模型及正常HepG2细胞模型,以噻唑兰染色吸光度(MTT值)、油红O染色、甘油三酯(TG)值、总胆固醇(TC)值、脂蛋白脂酶(LPL)、肝脂酶(HL)活性、葡萄糖含量、肝糖原含量、己糖激酶(HK)活性评价不同分子质量黑木耳发酵产物对HepG2细胞脂质代谢及糖代谢的影响。结果:黑木耳发酵产物能够调节HepG2细胞脂质代谢及糖代谢,降低细胞脂肪积累及葡萄糖含量,提升脂肪代谢酶与糖代谢酶的活性。其中100~300 ku与>300 ku的黑木耳发酵产物作用效果更明显。结论:黑木耳发酵产物能够调节HepG2细胞脂质代谢及糖代谢,且100~300 ku与>300 ku黑木耳发酵产物作用效果更明显。

Objective:The effects of different molecular weight Auricularia auricular fermentation products on glucose lipid metabolism of HepG2 cells were evaluated by establishing HepG2 cell fat accumulation model and normal HepG2 cell model.The effects and differences of glucose lipid metabolism in different molecular weights of A.auricular were revealed.Methods:The supernatant of A.auricular fermentation was divided into 0-10,10-50,50-100,100-300 ku and>300 ku by ultrafiltration centrifuge tube,and the fermentation products of A.auricular was obtained by constant temperature drying.The HepG2 cell fat accumulation model and the normal HepG2 cell model was established.And the effects of different molecular weights fermentation products of A.auricular on glucose lipid metabolism were evaluated by thiazolyl staining absorbance(MTT value),oil red O staining,triglyceride(TG)value,total cholesterol(TC)value,lipoprotein lipase(LPL),Hepatic lipase(HL)activity,glucose content,hepatic glycogen content and hexokinase(HK)activity.Results:The fermentation products of A.auricular can regulate the glucose lipid metabolism of HepG2 cells,reduce cell fat accumulation and glucose content,and increase the activity of fat metabolism enzyme and sugar metabolism enzyme.Among them,the effects of 100-300 ku and>300 ku A.auricular fermentation products were more obvious.Conclusion:The fermentation products of A.auricular can regulate glucose lipid metabolism in HepG2 cells,and the effects of 100-300 ku and>300 ku A.auricular fermentation products are more obvious.