小麦网腥黑穗病温室内人工接种方法的探索

Exploring of the artificial inoculation methods of Tilletia caries

小麦网腥黑穗病是由小麦网腥黑粉菌Tilletia caries引起,会导致小麦严重减产。为了获得小麦网腥黑穗病较高的发病率,本研究利用穗部接菌、土壤接菌、根部接菌3种方法进行温室内人工接种小麦。结果表明,利用穗部接菌及土壤接菌发病率分别为52.5%和10%,而根部接菌发病率仅有4%。利用穗部接菌可以获得更多的发病植株,为更好地研究寄主与病原的互作关系奠定了基础。

Tilletia caries can lead to common bunt of wheat and it can causes severe wheat yield reduction.In order to obtain the diseased plants of T.caries,three methods including tassel inoculation,soil inoculation and root inoculation were used to artificially inoculate in greenhouse.The results showed that disease incidence with tassel inoculation and soil inoculation was 52.5%and 10%,but the disease incidence with root inoculation was only 4%.More diseased plants can be obtained by using tassel inoculation,which lays a foundation for better research on the interaction between the host and the pathogen.