量子点荧光免疫检测牛奶中己烯雌酚、雌二醇

Quantum Dots Fluorescence Immunoassay Technology to Detect Diethylstilbestrol and 17β-estradiol Residues in Milk

研究牛奶中己烯雌酚、雌二醇的荧光免疫检测技术并对实际样品进行检测。辅助量子点标记技术,结合间接竞争酶联免疫吸附法原理建立检测标准曲线,评价特异性,对实际样品进行检测。己烯雌酚的荧光免疫检测在0.472 ng/mL~66.597 ng/mL内呈线性关系,最低检测限为0.236 ng/mL。雌二醇在0.418 ng/mL~195.065 ng/mL内呈线性关系,最低检测限为0.109 ng/mL。建立一种快速检测牛奶中己烯雌酚的荧光免疫检测方法,方法可同时运用于雌二醇的检测,特异性与回收率良好,可补充传统方法用于实际样品中雌激素类残留测定。

The fluorescence immunoassay of diethylstilbestrol and 17β-estradiol in milk was studied and the samples was detected.The standard curve was established with the aid of quantum dot labeling technology and the principle of indirect competitive enzyme linked immunosorbent assay to evaluate the specificity and detect the actual samples.The fluorescence immunoassay of diethylstilbestrol showed a linear relationship within 0.472 ng/mL-66.597 ng/mL,and the minimum detection limit was 0.236 ng/mL.The 17β-estradiol showed a linear relationship within 0.418 ng/mL-195.065 ng/mL,and the minimum detection limit was 0.109 ng/mL.A rapid fluorescence immunoassay method for the detection of diethylstilbestrol in milk was established,which could be used for the detection of 17β-estradiol at the same time with good specificity and recovery,and could be used to supplement the traditional method for the determination of estrogen residues in actual samples.