V8 Nexus高速自动毛细管电泳仪血清蛋白电泳参考区间的建立

Establishment of reference interval for the detection of serum protein electrophoresis by V8 Nexus high speed automated capillary electrophoresis apparatus

目的初步建立V8 Nexus高速自动毛细管电泳仪(简称V8毛细管电泳仪)血清蛋白电泳(SPE)的参考区间。方法选取表面健康者649名,按年龄分为18~29岁组、30~39岁组、40~49岁组、50~59岁组及≥60岁组。采用V8毛细管电泳仪[毛细管电泳(CE)法]检测所有对象的血清样本,依据我国卫生行业标准WS/T 402—2012《临床实验室检验项目参考区间的制定》建立SPE的参考区间。结果男、女性所有数据均服从正态分布或近似正态分布(P>0.05)。男、女性之间白蛋白、α2球蛋白、β1球蛋白和γ球蛋白差异均有统计学意义(P<0.05),α1球蛋白、β2球蛋白和β球蛋白结果不同性别之间差异均无统计学意义(P>0.05)。男性18~59岁各组白蛋白、α2球蛋白、β1球蛋白与≥60岁组比较,差异均有统计学意义(P>0.05),18~59岁各组之间差异均无统计学意义(P>0.05),而α1球蛋白、β2球蛋白、γ球蛋白和β球蛋白各年龄组之间差异均无统计学意义(P>0.05)。女性18~59岁各组白蛋白、β1球蛋白、β2球蛋白、β球蛋白与≥60岁组比较,差异均有统计学意义(P>0.05),18~59岁各组之间差异均无统计学意义(P>0.05),而α1球蛋白、α2球蛋白和γ球蛋白各年龄组之间差异均无统计学意义(P>0.05)。依据LSD检验、SNK检验和Z检验结果,可将各年龄组合并为一组,建立SPE的参考区间。结论初步建立了V8毛细管电泳仪(CE法)SPE的参考区间。对于SPE而言,各临床实验室应根据实际情况选择是否按性别或年龄分层建立参考区间。

Objective To preliminarily establish a reference interval for serum protein electrophoresis(SPE)by V8 Nexus high speed automated capillary electrophoresis(CE)apparatus.Methods A total of 649 healthy subjects were enrolled in this study and classified into 5 groups according to ages,including 18-29-year-old,30-39-year-old,40-49-year-old,50-59-year-old and≥60-year-old groups.Serum samples were detected by the CE apparatus.The reference interval of CE for SPE detection was evaluated according to the document method of the Health Industry Standard WS/T 402—2012 of the People's Republic of China.Results All data from males and females obeyed normal distribution or approximately normal distribution(P>0.05).There were statistical differences in the levels of albumin,α2 globulin,β1 globulin andγglobulin between males and females(P<0.05),while there was no statistical significance in the results ofα1 globulin,β2 globulin orβglobulin between males and females(P>0.05).In males,there were statistical differences in the levels of albumin,α2 globulin andβ1 globulin between 18-59-year-old groups and≥60-year-old group(P<0.05),but there was no statistical significance in these parameters between 18-59-year-old groups(P>0.05);neither wereα1 globulin,β2 globulin,γglobulin orβglobulin between any groups(P>0.05).In females,there were statistical differences in the levels of albumin,β1 globulin,β2 globulin andβglobulin between 18-59-year-old groups and≥60-year-old group(P<0.05),but there was no statistical significance in these parameters between 18-59-year-old groups(P>0.05);neither wereα1 globulin,α2 globulin orγglobulin between any groups(P>0.05).According to the results of LSD test,SNK test and Z test,the age groups could be combined into one group to establish the reference interval of SPE.Conclusion The reference interval of SPE has been preliminarily established by V8 Nexus high speed automated CE apparatus.Clinical laboratories should establish a reference interval for different sexes or ages according to actual situation.