沙眼衣原体DNA质控物的制备及评价

Preparation and evaluation of the quality control materials of Chlamydia trachomatis DNA

目的制备沙眼衣原体(CT)DNA质控物,并评价其稳定性。方法利用混合分泌物样本制备弱阳性和阳性质控物。采用37℃水浴和室温(22℃)2种融解方式对质控物融解稳定性进行评价;应用一元线性回归分析对质控物冷冻保存稳定性进行评价。结果在融解方式对质控物稳定性评价方面,弱阳性质控物评价结果差异有统计学意义(P<0.05),而阳性质控物评价结果差异无统计学意义(P>0.05);在冷冻保存稳定性评价方面,弱阳性和阳性质控物线性回归分析结果显示,质控物随保存时间的延长均没有显著性趋向变化(P>0.05)。结论CT DNA阳性质控物具有制备简便、稳定性好、成本低等优点,可以应用于实验室检测。实验室冷冻保存的分泌物样本采取37℃水浴融解方式稳定性最好。

Objective To prepare quality control materials of Chlamydia trachomatis(CT)DNA,and to evaluate its stability.Methods The weak positive and positive quality control materials wereprepared with pooled secretion samples.The melting stability of the quality control materials was evaluatedby water bath at 37℃and room temperature(22℃).The stability of the quality control materials in freezing storage was evaluated using univariate linear regression model.Results The melting stability evaluation for the weak positive control material showed acceptable results with significant differences(P<0.05),but positive control material showed acceptable results without significant differences(P>0.05).The cryopreserved stability evaluation for the weak positive and positive control materials showed that no significant linear trend was found with extended test time(P>0.05).Conclusion Positive quality control materials of CT DNA have advantages of simple preparation,good stability and low cost,and can be used in laboratory detection.The cryopreserved secretion samples in the laboratory can achieve the beststability when they are melted in 37℃water bath.