MiR-128-3p靶向抑制HOXA5调控多形性胶质母细胞瘤的增殖、侵袭与凋亡

MiR-128-3p Regulates Proliferation,Migration and Apoptosis of Glioblastoma Multiforme by Targeting HOXA5

目的探索HOXA5在胶质母细胞瘤(GBM)中高表达的原因及miR-128-3p调控胶质母细胞瘤进展的分子机制。方法通过慢病毒转染上调或下调U87细胞中miR-128-3p的表达水平,再利用蛋白质免疫印迹法检测HOXA5的表达水平的变化来探索miR-128-3p与HOXA5在GBM中表达的相关性。利用双荧光素报告基因实验验证miR-128-3p对HOXA5的靶向抑制关系。利用miR-128-3p与HOXA5过表达的质粒转染U87细胞进行拯救实验,通过CCK-8、Transwell、流式细胞学分析与裸鼠体内实验验证miR-128-3p调控GBM增殖、侵袭及凋亡方面的分子机制。结果上调U87细胞中miR-128-3p表达后HOXA5的表达水平显著下降,下调U87细胞中miR-128-3p表达后HOXA5表达水平明显升高(P<0.05),两者表达呈显著负相关。miR-128-3p可靶向结合HOXA5基因的3’UTR区并抑制HOXA5表达。miR-128-3p+Control组U87细胞增殖、侵袭及抗凋亡能力显著下降。结论miR-128-3p可通过靶向抑制HOXA5负向调控GBM细胞的增殖、侵袭及抗凋亡能力,HOXA5在GBM中呈高表达与miR-128-3p表达水平降低有关。

Objective To investigate the reasons of HOXA5 overexpression in GBM and the molecular mechanism of miR-128-3p regulating the proliferation,invasion and apoptosis of glioblastoma multiforme.Methods After increasing and decreasing miR-128-3p expression in U87 cell lines by lentivirus transfection,the changes of HOXA5 expression were detected by Western blot,to explore the correlation between miR-128-3p and HOXA5 in GBM.The dual-luciferase reporter tests were performed to detect the target interaction of miR-128-3p with HOXA5.Through CCK-8 test,Transwell test,flow cytometric assay and tumor cell xenograft in nude mice,we verified molecular mechanism of miR-128-3p regulating the proliferation,invasion and apoptosis of GBM in vitro and in vivo.Results The expression level of HOXA5 was decreased in U87 cell line after miR-128-3p upregulation.In addition,the expression level of HOXA5 was increased in U87 cell line after miR-128-3p downregulation(P<0.05).The expression level of HOXA5 was correlated negatively with the expression of miR-128-3p in U87 cell lines.MiR-128-3p targetedly interacted with 3’UTR of HOXA5 and inhibited the expression of HOXA5.The proliferation,invasion and anti-apoptosis of U87 cells were significantly decreased in the miR-128-3p+control group.Conclusion MiR-128-3p regulates negatively the proliferation,invasion and anti-apoptosis of GBM cells by targeting HOXA5.The overexpression of HOXA5 is induced by downregulation of miR-128-3p in GBM.