橘皮汤降逆止呕作用机制的网络药理学分析

Network Pharmacology Analysis on the Mechanism of Adjusting Reversed Flow of Qi and Prevent Vomiting by Jupi Decoction

目的基于网络药理学研究橘皮汤治疗呕吐的作用机制。方法利用TCMSP数据库、DTPS靶点预测系统,以及Genecard、DisGeNET、TTD疾病数据库,筛选橘皮汤治疗呕吐的活性成分及其靶点,并借助Cytoscape软件构建药物-活性成分-靶点网络;应用STRING平台构建靶蛋白相互作用(PPI)网络;通过BioGPS数据库对潜在靶点进行组织器官定位分析;利用DAVID平台和Omicshare数据库对潜在靶点进行GO富集分析和KEGG富集分析。结果共获得橙皮素、川陈皮素、柠檬烯、辛弗林、6-姜酚、姜烯、姜酮等24个橘皮汤活性成分,以及5-羟色胺受体、胆碱能受体、组胺受体、多巴胺受体、GABA受体等158个止呕靶点;涉及神经元的组成、神经元投射、原生质膜的组成、突触膜、突触膜固有成分、突触后膜固有成分等生物过程及细胞组成;主要调控多巴胺能神经突触、5-羟色胺能神经突触、胆碱能突触、GABA能神经突触等信号通路。结论橘皮汤能够多成分、多靶点、多器官、多通路降逆止呕,从对症和对因两个方面协同作用。

Objective The mechanism of Jupi decoction in treating vomiting was studied based on network pharmacology.Methods The TCMSP,DTPS target prediction system,Genecard,DisGeNET and TTD database were used to screen the active ingredients and targets of Jupi decoction for treating vomiting,and Cytoscape software was applied to construct the network of drug-active ingredients-targets.The STRING platform was used to construct protein-protein interaction network;and BioGPS database was used for organ location analysis of the targets.With the help of DAVID platformand Omicshare database,the GO and KEGG enrichment analysis of targets were carried out.Results Twenty-four main active components including hesperidin,nobiletin,limonene,synephmne,6-gingerol,zingiberene,zingerone and 158 antiemetic targets,such as 5-hydroxytryptamine receptor,cholinergic receptor,histamine receptor,dopamine receptor and GABA receptor,were obtained;involving the composition of neurons,neuronal projection,composition of protoplasmic membrane,synaptic membrane,intrinsic components of synaptic membrane,intrinsic components of postsynaptic membrane and other biological processes and cell composition;regulating signaling pathways included Dopaminergic synapse,5-hydroxy tiyptamine synapse,cholinergic synapse,GABAergic synapse.Conclusion Jupi decoction has multiple components,which have multiple targets,targeting multiple organs and multiple pathways,to adjust reversed flow of qi and prevent vomiting,from the two aspects on symptom and cause of vomiting,to synergistically exert the antiemetic effect.