广西玉林市规模化禽场禽流感病毒检测

Detection of Avian Influenza Virus in Scale Poultry Farms in Yulin City of Guangxi

为了解广西玉林市2020年规模禽场禽流感病毒感染状况,采用荧光RT-PCR方法,对广西玉林市7个县(市、区)42个规模化禽场采集的1260份禽喉/泄殖腔棉拭子样品进行了通用型禽流感病毒核酸检测(荧光PCR),并对检测为阳性的样本进行H5、H7亚型(双重荧光PCR)和H9亚型(荧光PCR)分型鉴定。结果显示:在42个规模化禽场中,未检出H5和H7亚型高致病性禽流感病毒阳性样品;在2个鸡场中检出18份H9亚型低致病性禽流感病毒阳性样品,在2个鸡场和4个鸭场中检出115份其他亚型低致病性禽流感病毒阳性样品。结果表明:在高致病性禽流感(H5+H7)三价灭活疫苗强制免疫政策下,广西玉林市规模化禽场的高致病性禽流感病毒感染风险较小,但仍须加强禽流感的免疫、监测,做好综合防控,以降低禽流感病毒由低致病性重组变异为高致病性的风险。本检测为指导广西玉林市禽流感防控提供了依据。

In order to identify the status of infection with avian influenza virus(AIV)in scale poultry farms in Yulin of Guangxi Province in 2020,1260 throat/cloacal swab samples collected from 42 scale poultry farms in 7 counties(cities,districts)were detected for AIV nucleic acids by fluorescent PCR,and the virus subtypes were identified by dual fluorescent PCR(H5 and H7 subtype)and fluorescent PCR(H9 subtype).The results showed that no positive sample infected with H5 and H7 subtype highly pathogenic avian influenza virus(HPAIV)was detected in all the farms;18 positive samples with H9 subtype low pathogenic avian influenza virus(LPAIV)were detected in two chicken farms,and 115 positive samples with other subtypes LPAIV were detected in two chicken farms and four duck farms.It was concluded that the risk of infection with HPAIV in scale farms in the city was lower due to the compulsory vaccination of HPAI(H5+H7)trivalent inactivated vaccines,but the vaccination and monitoring of avian influenza(AI)should be further strengthened to ensure comprehensive prevention and control,so as to reduce the risk of mutation of LPAIV into HPAIV.Some basis was provided for AI prevention and control in Yulin City.