口蹄疫病毒A型竞争ELISA抗体检测试剂盒在流行病学调查中的应用

Application of Solid-phase Competition ELISA for Detection of FMDV Type A Antibody in Epidemiological Investigation

为评价口蹄疫病毒A型竞争ELISA(cELISA)抗体检测试剂盒在流行病学调查中的应用前景,对2017年从福建省三明市采集的336份黄牛、奶牛、羊和猪血清样品,用A型cELISA抗体检测试剂盒进行抗体检测。结果显示,92份黄牛血清、92份羊血清、92份猪血清、60份奶牛血清的A型抗体阳性率分别为13.04%、11.96%、20.65%、86.67%。从上述4种血清中,各挑选10份血清(阴性、阳性各5份)共40份,采用口蹄疫病毒液相阻断ELISA(LPB-ELISA)抗体检测试剂盒进行验证。结果显示:cELISA检测为阳性的20份血清中,用LPB-ELISA检出阳性19份;cELISA检测为阴性的20份血清中,用LPB-ELISA检出阴性17份;两种方法的κ值为0.8,总符合率为90.00%。结果表明,A型cELISA试剂盒与LPB-ELISA试剂盒的符合率和一致性均较高,可用于口蹄疫流行病学调查和血清学监测。

In order to evaluate the application prospect of a novel solid-phase competition ELISA(cELISA)kit for detecting the antibody level of foot and mouth disease(FMDV)type A,a total of 336 clinical serum samples(92 cattle samples,92 sheep samples,92 pig samples and 60 cow samples)in Sanming City of Fujian Province were tested.The results showed that the positive rates of serum samples for cattle,sheep,pigs and cows were 13.04%,11.96%,20.65%and 86.67%,respectively,then 40 serum samples(10 for each kind of livestock samples,5 for each negative and positive samples)were selected to verify the above results by the liquid-phase-blocking ELISA(LPB-ELISA)kit.It was concluded that,for 20 positive samples by cELISA,19 were positive as detected by LPBELISA;for 20 negative samples by cELISA,17 were negative by LPB-ELISA;and theκvalue of the two kits was 0.8,with a total compliance rate of 90.00%,which indicated that the coincidence rate and consistency of the two kits were relatively high,and the cELISA kit for FMDV type A could be used in epidemiological investigation and serological surveillance of FMD.