貉细小病毒分离鉴定及其VP2基因序列分析

Isolation and Identification of Raccoon Dog Parvovirus and Sequence Analysis on Its VP2 Gene

本研究从山东省某貉养殖场发病貉粪便样品中分离到一株貉细小病毒(raccoon dog parvovirus,RDPV)。为了解该毒株的特性及其致病特点,对发病貉粪便处理后接种F81细胞,收获病毒液进行VP2基因序列分析、理化特性研究以及貉人工感染试验。结果显示:该分离株与RDPV参考株核苷酸同源性高达99.4%,属于犬细小病毒2型(CPV-2);对氯仿等不敏感,耐酸耐热,符合细小病毒特征;分离毒株对貉具有较强的致病力。结果表明,山东省仍存在RDPV流行且致病性有增强风险,须针对性研发RDPV疫苗,加强对RDPV流行的控制。本试验为RDPV流行病学研究提供了数据支持。

A strain of raccoon dog parvovirus(RDPV)was isolated from the feces of sick raccoon dogs in a farm in Shandong Province.In order to identify the characteristics of the isolate and its pathogenicity,virus suspension was harvested through inoculating the processed feces into F81 cells,and VP2 gene sequence analysis,physicochemical property study as well as artificial infection test were carried out.The results showed that the isolated strain belonged to canine parvovirus type 2(CPV-2),and its nucleotide homology with RDPV reference strain was up to 99.4%;it was insensitive to chloroform,but acid-resistant and heat-resistant,which conformed to the characteristics of parvovirus;the isolate was with strong pathogenicity to raccoon dogs.In conclusion,RDPV was still prevalent in Shandong Province with increasing risk of pathogenicity,and corresponding vaccines should be developed specifically to further control any virus spreading.As an outcome,epidemiological investigation on RDPV was supported with data provided in the study.