应用分子标记辅助选择培育抗稻瘟病恢复系R153

Marker-Assisted Selection for Hybrid Rice Restorer Line R153 Conferring Resistance to Blast

稻瘟病是危害水稻最严重的病害之一。培育抗稻瘟病的水稻品种是减少因病害损失的有效方法。本研究以携带稻瘟病抗性等位基因Pi25的品系BL108为供体、恢复系恢11-32为受体,应用该抗性基因的连锁标记Si13070C和功能标记CAP3/BglⅡ进行分子标记辅助选择,培育出携带Pi25抗性等位基因的恢复系R153,经福建省上杭县茶地国家水稻新品种稻瘟病抗性区试点鉴定,表现为抗稻瘟病。该恢复系的选育为杂交稻新组合选育提供新的亲本资源。

The rice blast is one of the most serious diseases in rice production.Breeding for disease resistant varieties is the most effective and efficient method to reduce the disease damage.In this study,one rice candidate restorer line,R153,harboring the rice blast resistance gene Pi25 was selected through the approach of molecular marker-assisted selection using segregating population derived from cross between the Hui11-32 and BL108(the donor of the blast resistance gene Pi25).Tightly linked DNA marker Si13070C and functional marker CAP3/BglⅡwere used.The blast resistance identification was conducted in a rice blast disease hotspot location,Shanghang County,Fujian Province,China.R153 showed a good resistance to rice blast in the evaluation and provided a new resource for rice breeding.