摘要
Two potent anti-MRSA tanshinone glycosides(1 and 2)were discovered by targeted microbial biotransformation,along with rapid identification via MS/MS networking.Serial reactions including dehydrogenation,demethylations,reduction,glycosylation and methylation have been observed after incubation of tanshinone IIA and fungus Mucor rouxianus AS 3.3447.In addition,tanshinosides B(2)showed potent activities against serial clinical isolates of oxacillin-resistant Staphylococcus aureus with MIC values of 0.78 mg/mL.This is the first study that shows a significant increase in the level and activities of tanshinone glycosides relative to the substrate tanshinone IIA.
基金
the National Program on Key Basic Research Project(973 program,2013CB734000)
by grants from the China Ocean Mineral Resources R&D Association(DY125-15-T-07)
the National Natural Science Foundation of China(81573341,81102369,81302678,31430002,31400090,31320103911,31125002)
the Ministry of Science and Technology of China(2013ZX10005004-005 and 2011ZX09102-011-11)
the European Union's Seventh Framework Programme(FP7/2007e2013)under grant agreement no.312184.
