摘要
Hydrogels are a class of materials that has the property of injectability and in situ gel formation.This property of hydrogels is manipulated in this study to develop a biomimetic bioresorbable injectable system of alginate nanobeads interspersed in fibrin network.Alginate nanobeads developed by calcium cross-linking yielded a size of 200e500 nm.The alginate nanobeads fibrin hydrogel was formed using dual syringe apparatus.Characterization of the in situ injectable hydrogel was done by SEM,FTIR and Rheometer.The developed hydrogel showed mechanical strength of 19 kPa which provides the suitable compliance for soft tissue engineering.Cytocompatibility studies using human umbilical cord blood derived mesenchymal stem cells showed good attachment,proliferation and infiltration within the hydrogel similar to fibrin gel.The developed in situ forming hydrogel could be a suitable delivery carrier of stem cells for soft tissue regeneration.
基金
One of the authors R.Jayakumar is grateful to the Department of Biotechnology(DBT),India,for providing fund under the scheme of Nanoscience and Nanotechnology Program(Ref.No.BT/PR 13585/NNT/28/474/2010)
One of the author S.Deepthi is thankful to the Council of Scientific and Industrial Research for supporting financially under the CSIR-SRF award no:9/963(0034)2K13-EMR-I.
