富含脯氨酸蛋白11表达与胆管癌患者临床病理特征及预后的关系

Relationship between the expression of proline-rich protein 11 and the clinicopathological characteristics and prognosis in patients with cholangiocarcinoma

目的探究富含脯氨酸蛋白11(PRR11)表达与胆管癌患者临床病理特征及预后的关系。方法前瞻性选取2016年1月到2017年8月长沙医学院附属第一医院收治的87例行手术切除的胆管癌组织作为肿瘤组,另选35例正常胆管上皮组织作为对照组。采用免疫组化检测肿瘤组和对照组的组织中PRR11表达,并分析其与胆管癌患者临床病理及预后关系。结果免疫组化结果显示,肿瘤组胆管组织中PRR11蛋白表达阳性率(52.87%)高于对照组(5.71%),差异有统计学意义(P<0.05)。临床分期Ⅲ~Ⅳ期患者PRR11表达阳性率(75.86%)高于Ⅰ~Ⅱ期患者(41.38%),肿瘤中低分化患者PRR11表达阳性率(61.40%)高于高分化患者(36.67%),淋巴结转移患者PRR11表达阳性率(66.67%)高于未转移患者(40.00%),差异有统计学意义(P<0.05)。采用Kaplan-Meier进行生存分析显示,PRR11阳性患者3年生存率21.74%(10/46)低于PRR11阴性患者的51.22%(21/41),差异有统计学意义(P<0.05);多因素COX回归分析显示,临床分期、淋巴结转移、PRR11表达是影响胆管癌患者预后的独立因素(P<0.05)。结论PRR11在胆管癌组织中呈高表达,且与患者临床病理、不良预后密切相关。

Objective To explore the relationship between the expression of proline-rich protein 11(PRR11)and the clinicopathological characteristics and prognosis in patients with cholangiocarcinoma.Methods Eighty-seven cases of cholangiocarcinoma tissues undergoing surgical resection in the First Affiliated Hospital of Changsha Medical College were prospectively selected as the tumor group from January 2016 to August 2017,and another 35 cases of normal bile duct epithelial tissue were selected as the control group.The expression of PRR11 in the tissue of tumor group and control group was detected by immunohistochemistry and its relationship with the clinicopathology and prognosis in patients with cholangiocarcinoma was analyzed.Results The results of immunohistochemistry showed that the positive rate of PRR11 protein expression in the bile duct tissue of the tumor group was higher than that of the control group(52.87%vs.5.71%,P<0.05).The positive rate of PRR11 expression in patients with clinical stages III~IV was higher than those of stageⅠ~Ⅱ(75.86%vs.41.38%).The positive rate of PRR11 expression in patients with poorly differentiated tumors was higher than those of well-differentiated(61.40%vs.36.67%).The positive rate of PRR11 expression in patients with lymph node metastasis was higher than that of non-metastatic ones(66.67%vs.40.00%,P<0.05).The survival analysis by Kaplan-Meier showed that the 3-year survival rate of PRR11-positive patients was lower than that of PRR11-negative patients[21.74(10/46)vs.51.22%(21/41),P<0.05].Multivariate COX regression analysis showed that clinical staging,lymph node metastasis,and PRR11 expression were independent factors affecting the prognosis of patients with cholangiocarcinoma(P<0.05).Conclusion PRR11 is highly expressed in cholangiocarcinoma tissue and is closely related to the clinical pathology and poor prognosis of patients.