基于高通量测序的菟丝子生防菌“鲁保一号”转录组学研究

Transcriptome Analysis of Biological Control Agent Lubao 1 to Cuscuta chinensis Based on High-Throughput RNA-Seq Technology

“鲁保一号”是对菟丝子具有良好防效的生防菌,由于其致病力退化和分子遗传信息的匮乏,对于该菌的深入研究受到了限制。为进一步挖掘“鲁保一号”菌株的遗传资源,获得致病力退化相关的功能基因,本研究运用新一代高通量测序技术(Illumina Hiseq 2500)对“鲁保一号”菌株进行转录组测序分析。结果表明,获得了总长度为31126662 bp的unigene序列信息;对序列进行组装拼接后获得17031条单基因簇(unigene),平均长度为1827.65 bp。序列同源性比较表明,86.04%的序列与已知炭疽菌序列有不同程度的同源性。将unigene与KOG数据库进行比对,根据其功能大致分为25类,其中注释最多的是一般功能预测类基因,其次是翻译后修饰、蛋白折叠和分子伴侣类基因。针对测序得到的基因信息,筛选获得6个CDC相关基因,分为3个亚组。本研究通过对“鲁保一号”菌株进行高通量测序,获得了丰富的转录组信息,为新基因的挖掘和基因组学的研究奠定了基础。

Lubao 1 is a kind of microbial agent with high bio-herbicidal activity to Cuscuta chinensis.But its application and research has been limited because of pathogenicity degradation and lack of molecular genetic information.The transcriptome of Lubao 1 strain was sequenced using an Illumina Hiseq 2500 platform.A total of 31126662 bp sequence with unigene information was obtained,and a total of 17031 unigenes were assembled by initial sequence splicing;the average length of single unigene was 1827.65 bp.The sequence homology analysis revealed that 84.06%of these unigenes had significant matches with Colletotrichum.The unigenes were further annotated based on KOG category,which could be grouped into 25 functional categories,among which,the most annotated was the general function prediction only ones,followed by post-translational modification,protein turnover and chaperones genes.Six cell division cycle genes were obtained by transcriptome analysis,and belonged to 3 branches.By high-throughput sequencing,we obtained abundant transcriptome information that could contribute to novel gene identification and genome research in Lubao 1.